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Stem-Loop Binding Protein (SLBP) (AA 1-271) antibody

Details for Product No. ABIN394132
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Antigen
Synonyms CG11886, Dmel\\CG11886, SLBP, dSLBP, dSLPB, slbp, cb157, sb:cb157, si:dkey-102m7.2, GB13630, HBP, slbp1, xslbp
Epitope
AA 1-271
(6), (1), (1), (1), (1)
Reactivity
Human
(13), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(8), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(14), (10), (6), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN394132
450.00 $
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Immunogen SLBP (AAH14908, 1 a.a. ~ 271 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C4-1C8
Isotype IgG2a kappa
Specificity SLBP (AAH14908, 1 a.a. ~ 271 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SLBP
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 31286 DA
Gene ID 12406
Research Area Translation Factors, Chromatin, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Stem-Loop Binding Protein (SLBP) (AA 1-271) antibody Immunofluorescence of monoclonal antibody to SLBP on HeLa cell.[antibody concentration 10 µg/mL] SLBP monoclonal antibody (M01), clone 2C4-1C8 Western Blot analysis of SLBP expression in Hela NE.
anti-Stem-Loop Binding Protein (SLBP) (AA 1-271) antibody (2) anti-Stem-Loop Binding Protein (SLBP) (AA 1-271) antibody (Image 2)
Background publications Narita, Yung, Yamamoto et al.: "NELF interacts with CBC and participates in 3' end processing of replication-dependent histone mRNAs." in: Molecular cell, Vol. 26, Issue 3, pp. 349-65, 2007 (PubMed).

Cakmakci, Lerner, Wagner et al.: "SLIP1, a factor required for activation of histone mRNA translation by the stem-loop binding protein." in: Molecular and cellular biology, Vol. 28, Issue 3, pp. 1182-94, 2008 (PubMed).

Mullen, Marzluff: "Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'." in: Genes & development, Vol. 22, Issue 1, pp. 50-65, 2008 (PubMed).

Koseoglu, Graves, Marzluff: "Phosphorylation of threonine 61 by cyclin a/Cdk1 triggers degradation of stem-loop binding protein at the end of S phase." in: Molecular and cellular biology, Vol. 28, Issue 14, pp. 4469-79, 2008 (PubMed).

Sullivan, Mullen, Marzluff et al.: "Knockdown of SLBP results in nuclear retention of histone mRNA." in: RNA (New York, N.Y.), Vol. 15, Issue 3, pp. 459-72, 2009 (PubMed).

Validation Images
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