DPH1 Homolog (S. Cerevisiae) (DPH1) antibody

Details for Product No. ABIN394133
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Synonyms Ovca1, Dph2l1, AW551873, 2310011M22Rik, 4930488F09Rik, RGD1562694, DPH1-OVCA2, DPH2L, DPH2L1, OVCA1, zgc:110702, dph1-ovca2, dph2l, dph2l1, ovca1, DPH1
(25), (15), (15), (13), (13), (1), (1), (1)
(25), (2)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Western Blotting (WB)
(18), (10), (7), (3), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN394133
Quantity 0.1 mg
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Immunogen DPH1 (NP_001374, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C5
Isotype IgG2b kappa
Specificity DPH1 (NP_001374, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DPH1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 48833 DA
Gene ID 12406
UniProt NP_001374
Research Area Translation Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000

Background: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DPH1 Homolog (S. Cerevisiae) (DPH1) antibody Immunofluorescence of monoclonal antibody to DPH1 on HeLa cell (antibody concentration 10 µg/mL). DPH1 monoclonal antibody (M02), clone 2C5 Western Blot analysis of DPH1 expression in HeLa.
anti-DPH1 Homolog (S. Cerevisiae) (DPH1) antibody (2) anti-DPH1 Homolog (S. Cerevisiae) (DPH1) antibody (Image 2)
Background publications Chen, Behringer: "Cloning, structure, and expression of the mouse Ovca1 gene." in: Biochemical and biophysical research communications, Vol. 286, Issue 5, pp. 1019-26, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Cardoso, Leventer, Ward et al.: "Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3." in: American journal of human genetics, Vol. 72, Issue 4, pp. 918-30, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Liu, Milne, Kuremsky et al.: "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9487-97, 2004 (PubMed).

Hosts (25), (2)
Reactivities (25), (15), (15), (13), (13), (1), (1), (1)
Applications (18), (10), (7), (3), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (1), (1), (1), (1)
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