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DPH1 Homolog (S. Cerevisiae) (DPH1) antibody

Details for Product No. ABIN394133
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Antigen
Synonyms Ovca1, Dph2l1, AW551873, 2310011M22Rik, 4930488F09Rik, RGD1562694, DPH1-OVCA2, DPH2L, DPH2L1, OVCA1, zgc:110702, dph1-ovca2, dph2l, dph2l1, ovca1, DPH1
Reactivity
Human
(38), (15), (15), (13), (13), (1), (1), (1)
Host
Mouse
(31), (9)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(31), (20), (10), (7), (3), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN394133
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen DPH1 (NP_001374, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C5
Isotype IgG2b kappa
Specificity DPH1 (NP_001374, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DPH1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 48833 DA
Gene ID 12406
UniProt NP_001374
Research Area Translation Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DPH1 Homolog (S. Cerevisiae) (DPH1) antibody Immunofluorescence of monoclonal antibody to DPH1 on HeLa cell (antibody concentration 10 µg/mL). DPH1 monoclonal antibody (M02), clone 2C5 Western Blot analysis of DPH1 expression in HeLa.
anti-DPH1 Homolog (S. Cerevisiae) (DPH1) antibody (2) anti-DPH1 Homolog (S. Cerevisiae) (DPH1) antibody (Image 2)
Background publications Chen, Behringer: "Cloning, structure, and expression of the mouse Ovca1 gene." in: Biochemical and biophysical research communications, Vol. 286, Issue 5, pp. 1019-26, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Cardoso, Leventer, Ward et al.: "Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3." in: American journal of human genetics, Vol. 72, Issue 4, pp. 918-30, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Liu, Milne, Kuremsky et al.: "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9487-97, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN394133
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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