DPH1 Homolog (S. Cerevisiae) (DPH1) antibody

Antigen: DPH1 Homolog (S. Cerevisiae) (DPH1)

Synonyms: Ovca1, Dph2l1, AW551873, 2310011M22Rik, 4930488F09Rik, RGD1562694, DPH1, DPH1-OVCA2, MGC128661, DPH2L, OVCA1, DPH2L1, FLJ33211, zgc:110702

Clonality: Monoclonal (2C5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394133

Additional Information

Alternative name: DPH1

Gene ID: 1801

UniProt: NP_001374

Immunogen: DPH1 (NP_001374, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2C5

Description: Other names: DPH2L, DPH2L1, FLJ33211, OVCA1 DPH1 homolog (S. cerevisiae)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DPH1 (NP_001374, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 48833 DA

Comments:

Background: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to DPH1 (AT1812a) on HeLa cell . [antibody concentration 10 ug/ml] DPH1 monoclonal antibody (M02), clone 2C5 Western Blot analysis of DPH1 expression in HeLa ( Cat # L013V1 ).

anti-DPH1 Homolog (S. Cerevisiae) (DPH1) antibody (Image 2)

Publications

Product: Chen, Behringer: "Cloning, structure, and expression of the mouse Ovca1 gene." in: Biochemical and biophysical research communications, Vol. 286, Issue 5, pp. 1019-26, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Cardoso, Leventer, Ward et al.: "Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3." in: American journal of human genetics, Vol. 72, Issue 4, pp. 918-30, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Liu, Milne, Kuremsky et al.: "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9487-97, 2004 (PubMed).