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DPH1 antibody (DPH1 Homolog (S. Cerevisiae)) (AA 216-325)

Details for Product anti-DPH1 Antibody No. ABIN394133, Supplier: Log in to see
Antigen
  • 2310011M22Rik
  • 4930488F09Rik
  • AW551873
  • DPH1
  • dph1-ovca2
  • DPH1-OVCA2
  • dph2l
  • DPH2L
  • Dph2l1
  • DPH2L1
  • dph2l1
  • Ovca1
  • ovca1
  • OVCA1
  • RGD1562694
  • zgc:110702
Epitope
AA 216-325
16
15
7
4
2
2
2
1
1
1
Reactivity
Human
59
18
16
1
1
1
1
1
Host
Mouse
49
10
Clonality (Clone)
Monoclonal ()
Conjugate
This DPH1 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
47
28
11
9
5
3
1
Supplier
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Immunogen DPH1 (NP_001374, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C5
Isotype IgG2b kappa
Specificity DPH1 (NP_001374, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DPH1 (DPH1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 48833 DA
Gene ID 12406
NCBI Accession NP_001374
Research Area Translation Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DPH1 antibody (DPH1 Homolog (S. Cerevisiae)) (AA 216-325) (ABIN394133) Immunofluorescence of monoclonal antibody to DPH1 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-DPH1 antibody (DPH1 Homolog (S. Cerevisiae)) (AA 216-325) (ABIN394133) anti-DPH1 Homolog (S. Cerevisiae) (DPH1) (AA 216-325) antibody (Image 2)
Background publications Liu, Milne, Kuremsky, Fink, Leppla: "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9487-97, 2004 (PubMed).

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Cardoso, Leventer, Ward, Toyo-Oka, Chung, Gross, Martin, Allanson, Pilz, Olney, Mutchinick, Hirotsune, Wynshaw-Boris, Dobyns, Ledbetter: "Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3." in: American journal of human genetics, Vol. 72, Issue 4, pp. 918-30, 2003 (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Chen, Behringer: "Cloning, structure, and expression of the mouse Ovca1 gene." in: Biochemical and biophysical research communications, Vol. 286, Issue 5, pp. 1019-26, 2001 (PubMed).