TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7) antibody

Antigen: TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7)

Synonyms: TAF2F, TAFII55, 55kDa, Taf2f, AI607308, BB007175, MGC86629, CHUNP6888, MGC174760

Clonality: Monoclonal (2C5)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394135

Additional Information

Alternative name: TAF7

Gene ID: 6879

Immunogen: TAF7 (AAH32737, 130 a.a. ~ 224 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2C5

Description: Other names: TAF2F, TAFII55 TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: TAF7 (AAH32737, 130 a.a. ~ 224 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 40259 DA

Comments:

Background: The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to TAF7 (AT4147a) on HeLa cell . [antibody concentration 10 ug/ml] Western blot analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi ( Cat # H00006879-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TAF7 monoclonal antibody (M01), clone 2C5 (Cat # AT4147a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7) antibody (Image 2)

Publications

Product: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Hartman, Walters, Hentosh: "Presence of the anti-leukemic nucleotide analog, 2-chloro-2'-deoxyadenosine-5'-monophosphate, in a promoter sequence alters DNA binding of TATA-binding protein (TBP)." in: Archives of biochemistry and biophysics, Vol. 459, Issue 2, pp. 223-32, 2007 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Gegonne, Weissman, Lu et al.: "TFIID component TAF7 functionally interacts with both TFIIH and P-TEFb." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 14, pp. 5367-72, 2008 (PubMed).