|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) antibody
|Synonyms||Ddx48, MGC6664, MGC6715, eIF4A-III, mKIAA0111, 2400003O03Rik, EIF4A3, MGC134221, DDX48, ddx48, nuk34, nmp265, eif4aiii, NMP265, NUK-34, hNMP265, KIAA0111, MGC10862, eIF4AIII|
Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||DDX48 (NP_055555, 312 a.a. ~ 412 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: KIAA0111, MGC10862, NUK34, eIF4A3, eIF4AIII, hNMP265 DEAD (Asp-Glu-Ala-Asp) box polypeptide 48|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||DDX48 (NP_055555, 312 a.a. ~ 412 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||46871 DA|
Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Immunohistochemistry ~~ 1.5ug/ml|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Translation Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).
Ma, Yoon, Richardson et al.: "SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs." in: Cell, Vol. 133, Issue 2, pp. 303-13, 2008 (PubMed).
Karam, Carvalho, Bruno et al.: "The NMD mRNA surveillance pathway downregulates aberrant E-cadherin transcripts in gastric cancer cells and in CDH1 mutation carriers." in: Oncogene, Vol. 27, Issue 30, pp. 4255-60, 2008 (PubMed).
Edelmann, Iphöfer, Akutsu et al.: "Structural basis and specificity of human otubain 1-mediated deubiquitination." in: The Biochemical journal, Vol. 418, Issue 2, pp. 379-90, 2009 (PubMed).
Koroleva, Calder, Pendle et al.: "Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxia." in: The Plant cell, Vol. 21, Issue 5, pp. 1592-606, 2009 (PubMed).
|Hosts||Rabbit (25), Mouse (3)|
|Reactivities||Human (27), Mouse (Murine) (12), Rat (Rattus) (12), Chicken (2), Cow (Bovine) (2), Dog (Canine) (2), Caenorhabditis elegans (C. elegans) (1), Cat (Feline) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (26), ELISA (17), Immunohistochemistry (IHC) (7), Immunofluorescence (IF) (6), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunoprecipitation (IP) (1)|
|Epitopes||Isoform 2 (7), Isoform 3 (7), N-Term (2), AA 176-411 (1), C-Term,AA 297-325 (1), Internal Region (1)|