Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) antibody

Antigen: Eukaryotic Translation Initiation Factor 4A3 (EIF4A3)

Synonyms: Ddx48, MGC6664, MGC6715, eIF4A-III, mKIAA0111, 2400003O03Rik, EIF4A3, MGC134221, DDX48, ddx48, nuk34, nmp265, eif4aiii, NMP265, NUK-34, hNMP265, KIAA0111, MGC10862, eIF4AIII

Clonality: Monoclonal (2D12)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Certificates: ISO 9001:2008

Catalog no.: ABIN394142

Additional Information

Alternative name: DDX48

Gene ID: 9775

UniProt: NP_055555

Immunogen: DDX48 (NP_055555, 312 a.a. ~ 412 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2D12

Description: Other names: KIAA0111, MGC10862, NUK34, eIF4A3, eIF4AIII, hNMP265 DEAD (Asp-Glu-Ala-Asp) box polypeptide 48

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DDX48 (NP_055555, 312 a.a. ~ 412 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 46871 DA

Comments:

Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to DDX48 ( Cat # AT1738a ) on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1 ug/ml] Detection limit for recombinant GST tagged DDX48 is approximately 0.03ng/ml as a capture antibody.

anti-Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) antibody (Image 2)

Publications

Product: Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Ma, Yoon, Richardson et al.: "SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs." in: Cell, Vol. 133, Issue 2, pp. 303-13, 2008 (PubMed).

Karam, Carvalho, Bruno et al.: "The NMD mRNA surveillance pathway downregulates aberrant E-cadherin transcripts in gastric cancer cells and in CDH1 mutation carriers." in: Oncogene, Vol. 27, Issue 30, pp. 4255-60, 2008 (PubMed).

Edelmann, Iphöfer, Akutsu et al.: "Structural basis and specificity of human otubain 1-mediated deubiquitination." in: The Biochemical journal, Vol. 418, Issue 2, pp. 379-90, 2009 (PubMed).

Koroleva, Calder, Pendle et al.: "Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxia." in: The Plant cell, Vol. 21, Issue 5, pp. 1592-606, 2009 (PubMed).