BCL2-Associated Athanogene (BAG1) antibody

Antigen: BCL2-Associated Athanogene (BAG1)

Synonyms: RAP46

Clonality: Monoclonal (2D3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394144

Additional Information

Alternative name: BAG1

Gene ID: 573

UniProt: NP_004314

Immunogen: BAG1 (NP_004314, 241 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2D3

Description: Other names: BCL2-associated athanogene

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BAG1 (NP_004314, 241 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 38878 DA

Comments:

Background: The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Cell Structure, Signaling

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to BAG1 (AT1260a) on HeLa cell . [antibody concentration 35 ug/ml] Western Blot analysis of BAG1 expression in transfected 293T cell line by BAG1 monoclonal antibody (M02), clone 2D3. Lane 1: BAG1 transfected lysate(38.878 KDa). Lane 2: Non-transfected lysate.

anti-BCL2-Associated Athanogene (BAG1) antibody (Image 2)

Publications

Product: Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Zheng, Xu, Xing et al.: "Nuclear or cytoplasmic localization of Bag-1 distinctly correlates with pathologic behavior and outcome of gastric carcinomas." in: Human pathology, Vol. 41, Issue 5, pp. 724-36, 2010 (PubMed).

Deeg, Gralle, Sroka et al.: "BAG1 restores formation of functional DJ-1 L166P dimers and DJ-1 chaperone activity." in: The Journal of cell biology, Vol. 188, Issue 4, pp. 505-13, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).