DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y) (AA 1-81) antibody

Details for Product No. ABIN394152
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms DDX3Y, DBY, Ddx3y, 8030469F12Rik, D1Pas1-rs1, Dby
Epitope
AA 1-81
(13), (8), (6), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(50), (23), (15), (12), (12), (12)
Host
Mouse
(40), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(32), (27), (17), (10), (7), (6), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 8 to 11 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN394152
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen DDX3Y (NP_004651, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2D7
Isotype IgG1 kappa
Specificity DDX3Y (NP_004651, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DDX3Y
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 73154 DA
Gene ID 12406
NCBI Accession NP_004651
Research Area Translation Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y) (AA 1-81) antibody DDX3Y monoclonal antibody (M01), clone 2D7 Western Blot analysis of DDX3Y expression in HeLa on formalin-fixed paraffin-embedded human testis (antibody concentration 3 µg/mL).
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y) (AA 1-81) antibody (2) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y) (AA 1-81) antibody (Image 2)
Background publications Pope, Lee: "Yeast two-hybrid identification of prostatic proteins interacting with human sex hormone-binding globulin." in: The Journal of steroid biochemistry and molecular biology, Vol. 94, Issue 1-3, pp. 203-8, 2005 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Lardone, Parodi, Valdevenito et al.: "Quantification of DDX3Y, RBMY1, DAZ and TSPY mRNAs in testes of patients with severe impairment of spermatogenesis." in: Molecular human reproduction, Vol. 13, Issue 10, pp. 705-12, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Rosinski, Fujii, Mito et al.: "DDX3Y encodes a class I MHC-restricted H-Y antigen that is expressed in leukemic stem cells." in: Blood, Vol. 111, Issue 9, pp. 4817-26, 2008 (PubMed).

Validation Images
Did you look for something else?
back to top