DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y)

Synonyms: DBY, Dby, D1Pas1-rs1, 8030469F12Rik, DDX3Y, DKFZp469G1332, Ddx3y

Clonality: Monoclonal (2D7)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394152

Additional Information

Alternative name: DDX3Y

Gene ID: 8653

UniProt: NP_004651

Immunogen: DDX3Y (NP_004651, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2D7

Description: Other names: DBY DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DDX3Y (NP_004651, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 73154 DA

Comments:

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

DDX3Y monoclonal antibody (M01), clone 2D7 Western Blot analysis of DDX3Y expression in HeLa ( Cat # L013V1 ). Immunoperoxidase of monoclonal antibody to DDX3Y ( Cat # AT1735a ) on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, Y-Linked (DDX3Y) antibody (Image 2)

Publications

Product: Pope, Lee: "Yeast two-hybrid identification of prostatic proteins interacting with human sex hormone-binding globulin." in: The Journal of steroid biochemistry and molecular biology, Vol. 94, Issue 1-3, pp. 203-8, 2005 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Lardone, Parodi, Valdevenito et al.: "Quantification of DDX3Y, RBMY1, DAZ and TSPY mRNAs in testes of patients with severe impairment of spermatogenesis." in: Molecular human reproduction, Vol. 13, Issue 10, pp. 705-12, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Rosinski, Fujii, Mito et al.: "DDX3Y encodes a class I MHC-restricted H-Y antigen that is expressed in leukemic stem cells." in: Blood, Vol. 111, Issue 9, pp. 4817-26, 2008 (PubMed).