GATA Binding Protein 2 (GATA2) antibody

Antigen: GATA Binding Protein 2 (GATA2)

Synonyms: NFE1B, MGC2306, FLJ45948, GATA2, Gata-2, MGC129339, xgata2, xgata-2, MGC131004, GATA-2, NF-E1b, GATA transcription factor 2, T14P1.14, Gata2

Clonality: Monoclonal (2F12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394161

Additional Information

Alternative name: GATA2

Gene ID: 2624

Immunogen: GATA2 (AAH18988, 1 a.a. ~ 103 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG Mix kappa

Clone: 2F12

Description: Other names: MGC2306, NFE1B GATA binding protein 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: GATA2 (AAH18988, 1 a.a. ~ 103 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 50396 DA

Comments:

Background: This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to GATA2 (AT2159a) on HeLa cell . [antibody concentration 10 ug/ml] GATA2 monoclonal antibody (M03), clone 2F12 Western Blot analysis of GATA2 expression in Hela NE ( Cat # L013V3 ).

anti-GATA Binding Protein 2 (GATA2) antibody (Image 2)

Publications

Product: Dandona, Chen, Fan et al.: "The transcription factor GATA-2 does not associate with angiographic coronary artery disease in the Ottawa Heart Genomics and Cleveland Clinic GeneBank Studies." in: Human genetics, Vol. 127, Issue 1, pp. 101-5, 2010 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Fujiwara, OGeen, Keles et al.: "Discovering hematopoietic mechanisms through genome-wide analysis of GATA factor chromatin occupancy." in: Molecular cell, Vol. 36, Issue 4, pp. 667-81, 2009 (PubMed).

Shimahara, Yamakawa, Nishikata et al.: "Acetylation of lysine 564 adjacent to the C-terminal binding protein-binding motif in EVI1 is crucial for transcriptional activation of GATA2." in: The Journal of biological chemistry, Vol. 285, Issue 22, pp. 16967-77, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).