DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 41 (DDX41) (AA 523-623) antibody

Details for Product No. ABIN394166
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Antigen
Synonyms DDX41, ABS, 2900024F02Rik, AA958953, AI324246, wu:fb92e02, zgc:55896
Epitope
AA 523-623
(10), (8), (7), (4), (3), (1), (1), (1), (1)
Reactivity
Human
(33), (4), (4), (3), (2), (1)
Host
Mouse
(19), (13), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunofluorescence (IF), Western Blotting (WB)
(33), (18), (8), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394166
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Immunogen DDX41 (NP_057306, 523 a.a. ~ 623 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2F4
Isotype IgG1 kappa
Specificity DDX41 (NP_057306, 523 a.a. ~ 623 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DDX41
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 69838 DA
Gene ID 12406
NCBI Accession NP_057306
Research Area Translation Factors, Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Based on studies in Drosophila, the abstrakt gene is widely required during post-transcriptional gene expression.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 41 (DDX41) (AA 523-623) antibody Immunofluorescence of monoclonal antibody to DDX41 on HeLa cell (antibody concentration 10 µg/mL). Western Blot analysis of DDX41 expression in transfected 293T cell line by DDX41 monoclonal antibody (M01), clone 2F4. Lane 1: DDX41 transfected lysate(70 KDa). Lane 2: Non-transfected lysate.
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 41 (DDX41) (AA 523-623) antibody (2) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 41 (DDX41) (AA 523-623) antibody (Image 2)
Background publications Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Wu, Ma, Brown et al.: "Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening." in: Proteomics, Vol. 7, Issue 11, pp. 1775-85, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Validation Images
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