DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 41 (DDX41) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 41 (DDX41)

Synonyms: ABS, MGC8828, AA958953, AI324246, 2900024F02Rik, DDX41

Clonality: Monoclonal (2F4)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394166

Additional Information

Alternative name: DDX41

Gene ID: 51428

UniProt: NP_057306

Immunogen: DDX41 (NP_057306, 523 a.a. ~ 623 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2F4

Description: Other names: ABS, MGC8828 DEAD (Asp-Glu-Ala-Asp) box polypeptide 41

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DDX41 (NP_057306, 523 a.a. ~ 623 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 69838 DA

Comments:

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Based on studies in Drosophila, the abstrakt gene is widely required during post-transcriptional gene expression.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to DDX41 (AT1736a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of DDX41 expression in transfected 293T cell line by DDX41 monoclonal antibody (M01), clone 2F4. Lane 1: DDX41 transfected lysate(70 KDa). Lane 2: Non-transfected lysate.

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 41 (DDX41) antibody (Image 2)

Publications

Product: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Wu, Ma, Brown et al.: "Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening." in: Proteomics, Vol. 7, Issue 11, pp. 1775-85, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).