Helicase, Lymphoid-Specific (HELLS) antibody

Antigen: Helicase, Lymphoid-Specific (HELLS)

Synonyms: LSH, PASG, SMARCA6, FLJ10339, Nbla10143, HELLS, cb65, pasg, sb:cb65, sb:cb749, im:6911667

Clonality: Monoclonal (2G10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394178

Additional Information

Alternative name: HELLS

Gene ID: 3070

Immunogen: HELLS (AAH30963, 1 a.a. ~ 349 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2G10

Description: Other names: FLJ10339, LSH, Nbla10143, PASG, SMARCA6 helicase, lymphoid-specific

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: HELLS (AAH30963, 1 a.a. ~ 349 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 97074 DA

Comments:

Background: This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

HELLS monoclonal antibody (M04), clone 2G10 Western Blot analysis of HELLS expression in HeLa NE ( Cat # L013V3 ).

Publications

Product: Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Myant, Stancheva: "LSH cooperates with DNA methyltransferases to repress transcription." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 215-26, 2007 (PubMed).

Suzuki, Farrar, Yegnasubramanian et al.: "Stable knockdown of PASG enhances DNA demethylation but does not accelerate cellular senescence in TIG-7 human fibroblasts." in: Epigenetics : official journal of the DNA Methylation Society, Vol. 3, Issue 5, pp. 281-91, 2008 (PubMed).

Palmieri, Wilson, Iversen et al.: "Polymorphism in the IL18 gene and epithelial ovarian cancer in non-Hispanic white women." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 17, Issue 12, pp. 3567-72, 2008 (PubMed).

Zhou, Han, Li et al.: "Senescence delay and repression of p16INK4a by Lsh via recruitment of histone deacetylases in human diploid fibroblasts." in: Nucleic acids research, Vol. 37, Issue 15, pp. 5183-96, 2009 (PubMed).