TAR (HIV-1) RNA Binding Protein 2 (TARBP2) antibody

Antigen: TAR (HIV-1) RNA Binding Protein 2 (TARBP2)

Synonyms: LOQS, TRBP, TRBP1, TRBP2, Prbp, fj51e12, zgc:63778, wu:fj51e12, MGC124970, MGC82499, tarbp2, MGC139801, trbp, trbp1, trbp2, MGC97783, TARBP2

Clonality: Monoclonal (2G10)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394180

Additional Information

Alternative name: TARBP2

Gene ID: 6895

UniProt: NP_599150

Immunogen: TARBP2 (NP_599150, 141 a.a. ~ 251 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2G10

Description: Other names: TRBP, TRBP1, TRBP2 Tar (HIV-1) RNA binding protein 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: TARBP2 (NP_599150, 141 a.a. ~ 251 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 39073 DA

Comments:

Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

TARBP2 monoclonal antibody (M04), clone 2G10. Western Blot analysis of TARBP2 expression in Hela NE ( Cat # L013V3 ).

Publications

Product: Daniels, Melendez-Peña, Scarborough et al.: "Characterization of the TRBP domain required for dicer interaction and function in RNA interference." in: BMC molecular biology, Vol. 10, pp. 38, 2009 (PubMed).

Wang, Noland, Siridechadilok et al.: "Structural insights into RNA processing by the human RISC-loading complex." in: Nature structural & molecular biology, Vol. 16, Issue 11, pp. 1148-53, 2009 (PubMed).

Lau, Potter, Carragher et al.: "Structure of the human Dicer-TRBP complex by electron microscopy." in: Structure (London, England : 1993), Vol. 17, Issue 10, pp. 1326-32, 2009 (PubMed).

Wilker, Baccarelli, Suh et al.: "Black carbon exposures, blood pressure, and interactions with single nucleotide polymorphisms in MicroRNA processing genes." in: Environmental health perspectives, Vol. 118, Issue 7, pp. 943-8, 2010 (PubMed).

Boni, Zarate, Villa et al.: "Role of primary miRNA polymorphic variants in metastatic colon cancer patients treated with 5-fluorouracil and irinotecan." in: The pharmacogenomics journal, Vol. 11, Issue 6, pp. 429-36, 2011 (PubMed).