Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody

Antigen: Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2)

Synonyms: CCTB, 99D8.1, PRO1633, CCT-beta, MGC142074, MGC142076, TCP-1-beta, fa26a01, fc58a11, CHUNP6883, wu:fa26a01, wu:fc58a11, cct2, MGC89448, CCT2, MGC128852, Cctb, MGC94480

Clonality: Monoclonal (2G6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394185

Additional Information

Alternative name: CCT2

Gene ID: 10576

UniProt: NP_006422

Immunogen: CCT2 (NP_006422, 117 a.a. ~ 218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2G6

Description: Other names: 99D8.1, CCTbeta, CCTB, PRO1633, TCP1beta chaperonin containing TCP1, subunit 2 (beta)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CCT2 (NP_006422, 117 a.a. ~ 218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 57488 DA

Comments:

Background: The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to CCT2 (AT1427a) on A-431 cell . [antibody concentration 10 ug/ml] CCT2 monoclonal antibody (M01), clone 2G6 Western Blot analysis of CCT2 expression in A-431 ( Cat # L015V1 ).

anti-Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody (Image 2)

Publications

Product: Goudreault, DAmbrosio, Kean et al.: "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 1, pp. 157-71, 2009 (PubMed).

Abe, Yoon, Kubota et al.: "p90 ribosomal S6 kinase and p70 ribosomal S6 kinase link phosphorylation of the eukaryotic chaperonin containing TCP-1 to growth factor, insulin, and nutrient signaling." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 14939-48, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).

Fislová, Thomas, Graef et al.: "Association of the influenza virus RNA polymerase subunit PB2 with the host chaperonin CCT." in: Journal of virology, Vol. 84, Issue 17, pp. 8691-9, 2010 (PubMed).