TAF11 antibody (TATA Box Binding Protein Associated Factor 11) (AA 158-211)

Details for Product anti-TAF11 Antibody No. ABIN394190, Supplier: Log in to see
Antigen
  • 1110038O14Rik
  • 28kDa
  • AI115401
  • AL024207
  • AW552398
  • PRO214
  • TAF2I
  • TAFII28
  • MGC:15243
  • TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa
  • Taf11
  • TAF11
Epitope
AA 158-211
17
16
12
7
6
3
2
Reactivity
Human
54
6
5
2
2
1
1
1
Host
Mouse
30
25
Clonality (Clone)
Monoclonal ()
Conjugate
This TAF11 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
53
38
18
11
10
8
1
1
Options
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Immunogen TAF11 (NP_005634, 158 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2G9
Isotype IgG1 kappa
Specificity TAF11 (NP_005634, 158 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TAF11 (TAF11 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23307 DA
Gene ID 12406
NCBI Accession NP_005634
Research Area Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-TAF11 antibody (TATA Box Binding Protein Associated Factor 11) (AA 158-211) (ABIN394190) Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-TAF11 antibody (TATA Box Binding Protein Associated Factor 11) (AA 158-211) (ABIN394190) anti-TATA Box Binding Protein Associated Factor 11 (TAF11) (AA 158-211) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Guermah, Ge, Chiang, Roeder: "The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis." in: Molecular cell, Vol. 12, Issue 4, pp. 991-1001, 2003 (PubMed).

Mungall, Palmer, Sims, Edwards, Ashurst, Wilming, Jones, Horton, Hunt, Scott, Gilbert, Clamp, Bethel, Milne, Ainscough, Almeida, Ambrose, Andrews, Ashwell, Babbage, Bagguley, Bailey, Banerjee, Barker et al.: "The DNA sequence and analysis of human chromosome 6. ..." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003 (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Mengus, Gangloff, Carré, Lavigne, Davidson: "The human transcription factor IID subunit human TATA-binding protein-associated factor 28 interacts in a ligand-reversible manner with the vitamin D(3) and thyroid hormone receptors." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10064-71, 2000 (PubMed).