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WT1 antibody (Wilms Tumor 1) (AA 349-439)

Details for Product anti-WT1 Antibody No. ABIN394194, Supplier: Log in to see
Antigen
  • AWT1
  • CWT1
  • D630046I19Rik
  • EWS-WT1
  • GUD
  • gud
  • NPHS4
  • si:ch211-160j6.3
  • wagr
  • WAGR
  • WIT-2
  • wit-2
  • WT
  • wt-1
  • Wt-1
  • WT-1
  • wt1
  • WT1
  • WT33
  • wt33
  • XeWT1
  • xWT1
  • xwt1
  • XWT1a
  • XWT1b
  • zgc:152697
Epitope
AA 349-439
34
20
15
14
14
11
9
9
9
5
4
2
1
1
1
1
1
1
1
Reactivity
Human
219
45
30
2
1
1
1
1
1
Host
Mouse
109
95
15
Clonality (Clone)
Monoclonal ()
Conjugate
This WT1 antibody is un-conjugated
9
8
8
7
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
Application
Western Blotting (WB)
120
100
99
57
55
52
25
3
3
2
2
1
Supplier
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Immunogen WT1 (NP_000369, 349 a.a. ~ 439 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2H4
Isotype IgG2b kappa
Specificity WT1 (NP_000369, 349 a.a. ~ 439 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name WT1 (WT1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 49188 DA
Gene ID 12406
NCBI Accession NP_000369
Research Area Translation Factors, Cancer, Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-WT1 antibody (Wilms Tumor 1) (AA 349-439) (ABIN394194) WT1 monoclonal antibody (M01), clone 2H4 Western Blot analysis of WT1 expression in A...
Background publications Rocquain, Carbuccia, Trouplin, Raynaud, Murati, Nezri, Tadrist, Olschwang, Vey, Birnbaum, Gelsi-Boyer, Mozziconacci: "Combined mutations of ASXL1, CBL, FLT3, IDH1, IDH2, JAK2, KRAS, NPM1, NRAS, RUNX1, TET2 and WT1 genes in myelodysplastic syndromes and acute myeloid leukemias." in: BMC cancer, Vol. 10, pp. 401, 2010

Hollink, van den Heuvel-Eibrink, Zimmermann, Balgobind, Arentsen-Peters, Alders, Willasch, Kaspers, Trka, Baruchel, Creutzig, Pieters, Reinhardt, Zwaan: "No prognostic impact of the WT1 gene single nucleotide polymorphism rs16754 in pediatric acute myeloid leukemia." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 28, Issue 28, pp. e523-6; author reply e527-e528, 2010

Borbulevych, Do, Baker: "Structures of native and affinity-enhanced WT1 epitopes bound to HLA-A*0201: implications for WT1-based cancer therapeutics." in: Molecular immunology, Vol. 47, Issue 15, pp. 2519-24, 2010

Bachas, Schuurhuis, Hollink, Kwidama, Goemans, Zwaan, van den Heuvel-Eibrink, de Bont, Reinhardt, Creutzig, de Haas, Assaraf, Kaspers, Cloos: "High-frequency type I/II mutational shifts between diagnosis and relapse are associated with outcome in pediatric AML: implications for personalized medicine." in: Blood, Vol. 116, Issue 15, pp. 2752-8, 2010

Machuca, Benoit, Nevo, Tête, Gribouval, Pawtowski, Brandström, Loirat, Niaudet, Gubler, Antignac: "Genotype-phenotype correlations in non-Finnish congenital nephrotic syndrome." in: Journal of the American Society of Nephrology : JASN, Vol. 21, Issue 7, pp. 1209-17, 2010