DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54)

Synonyms: DP97, MGC2835, AI414901, 2410015A15Rik, mgc2835, MGC111908, chunp6913, zgc:111908, RGD1562539, DDX54, MGC132273

Clonality: Monoclonal (2H6)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394196

Additional Information

Alternative name: DDX54

Gene ID: 79039

UniProt: NP_076977

Immunogen: DDX54 (NP_076977, 778 a.a. ~ 882 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2H6

Description: Other names: DP97, MGC2835 DEAD (Asp-Glu-Ala-Asp) box polypeptide 54

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DDX54 (NP_076977, 778 a.a. ~ 882 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 98595 DA

Comments:

Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to DDX54 (AT1739a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody (M01), clone 2H6. Lane 1: DDX54 transfected lysate(98.6 KDa). Lane 2: Non-transfected lysate.

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) antibody (Image 2)

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Andersen, Lam, Leung et al.: "Nucleolar proteome dynamics." in: Nature, Vol. 433, Issue 7021, pp. 77-83, 2005 (PubMed).

Satoh, Nanri, Yamamura: "Rapid identification of 14-3-3-binding proteins by protein microarray analysis." in: Journal of neuroscience methods, Vol. 152, Issue 1-2, pp. 278-88, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Edelmann, Iphöfer, Akutsu et al.: "Structural basis and specificity of human otubain 1-mediated deubiquitination." in: The Biochemical journal, Vol. 418, Issue 2, pp. 379-90, 2009 (PubMed).