|+1 404 474 4654|
|+1 888 205 9894 (TF)|
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) antibody
|Synonyms||DP97, MGC2835, AI414901, 2410015A15Rik, mgc2835, MGC111908, chunp6913, zgc:111908, RGD1562539, DDX54, MGC132273|
Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||DDX54 (NP_076977, 778 a.a. ~ 882 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: DP97, MGC2835 DEAD (Asp-Glu-Ala-Asp) box polypeptide 54|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||DDX54 (NP_076977, 778 a.a. ~ 882 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||98595 DA|
Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Translation Factors, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).
Andersen, Lam, Leung et al.: "Nucleolar proteome dynamics." in: Nature, Vol. 433, Issue 7021, pp. 77-83, 2005 (PubMed).
Satoh, Nanri, Yamamura: "Rapid identification of 14-3-3-binding proteins by protein microarray analysis." in: Journal of neuroscience methods, Vol. 152, Issue 1-2, pp. 278-88, 2006 (PubMed).
Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).
Edelmann, Iphöfer, Akutsu et al.: "Structural basis and specificity of human otubain 1-mediated deubiquitination." in: The Biochemical journal, Vol. 418, Issue 2, pp. 379-90, 2009 (PubMed).
|Hosts||Rabbit (6), Mouse (3)|
|Reactivities||Human (7), Mouse (Murine) (3), Cow (Bovine) (1), Dog (Canine) (1), Rat (Rattus) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (8), ELISA (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunofluorescence (IF) (1)|
|Epitopes||C-Term (3), AA 625-675 (1), AA 800-850 (1)|