Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-84) antibody

Details for Product No. ABIN394200
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Antigen
Synonyms ID3, MOR136-6, fa56g03, tdsubc_2g3, wu:fa56g03, ik:tdsubc_2g3, xx:tdsubc_2g3, heir-1, xid3, xidia, Hlh462, Idb3, bHLHb25, HEIR-1
Epitope
AA 1-84
(16), (12), (7), (7), (6), (5), (2), (1), (1), (1), (1)
Reactivity
Human
(69), (20), (16), (13), (13), (13)
Host
Mouse
(47), (26)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(45), (23), (21), (15), (14), (10), (5), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394200
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Immunogen ID3 (NP_002158, 1 a.a. ~ 84 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2H8
Isotype IgG2a kappa
Specificity ID3 (NP_002158, 1 a.a. ~ 84 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ID3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 12969 DA
Gene ID 12406
NCBI Accession NP_002158
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-84) antibody Immunofluorescence of monoclonal antibody to ID3 on HeLa cell (antibody concentration 10 µg/mL). ID3 monoclonal antibody (M03), clone 2H8 Western Blot analysis of ID3 expression in HeLa.
anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-84) antibody (2) anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-84) antibody (Image 2)
Background publications Trabosh, Daher, Divito et al.: "UVB upregulates the bax promoter in immortalized human keratinocytes via ROS induction of Id3." in: Experimental dermatology, Vol. 18, Issue 4, pp. 387-95, 2009 (PubMed).

Higgins, Wong, Richner et al.: "Fibroblast growth factor 2 reactivates G1 checkpoint in SK-N-MC cells via regulation of p21, inhibitor of differentiation genes (Id1-3), and epithelium-mesenchyme transition-like events." in: Endocrinology, Vol. 150, Issue 9, pp. 4044-55, 2009 (PubMed).

Shuno, Tsuno, Okaji et al.: "Id1/Id3 knockdown inhibits metastatic potential of pancreatic cancer." in: The Journal of surgical research, Vol. 161, Issue 1, pp. 76-82, 2010 (PubMed).

McGeachie, Ramoni, Mychaleckyj et al.: "Integrative predictive model of coronary artery calcification in atherosclerosis." in: Circulation, Vol. 120, Issue 24, pp. 2448-54, 2009 (PubMed).

Doran, Lehtinen, Meller et al.: "Id3 is a novel atheroprotective factor containing a functionally significant single-nucleotide polymorphism associated with intima-media thickness in humans." in: Circulation research, Vol. 106, Issue 7, pp. 1303-11, 2010 (PubMed).

Validation Images
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