Vav 1 Oncogene (VAV1) antibody

Antigen: Vav 1 Oncogene (VAV1)

Synonyms: VAV, Vav, vav-T, vav1, MGC80607, VAV1

Clonality: Monoclonal (3A11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394203

Additional Information

Alternative name: VAV1

Gene ID: 7409

Immunogen: VAV1 (AAH13361, 681 a.a. ~ 791 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3A11

Description: Other names: VAV vav 1 oncogene

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: VAV1 (AAH13361, 681 a.a. ~ 791 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 98314 DA

Comments:

Background: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to VAV1 ( Cat# AT4503a ) on HeLa cell . [antibody concentration 10 ug/ml] VAV1 monoclonal antibody (M04), clone 3A11 Western Blot analysis of VAV1 expression in Jurkat ( Cat # L017V1 ).

anti-Vav 1 Oncogene (VAV1) antibody (Image 2)

Publications

Product: Yu, Martins, Li et al.: "Structural and energetic mechanisms of cooperative autoinhibition and activation of Vav1." in: Cell, Vol. 140, Issue 2, pp. 246-56, 2010 (PubMed).

Kim, Das, Gross et al.: "Synergistic signals for natural cytotoxicity are required to overcome inhibition by c-Cbl ubiquitin ligase." in: Immunity, Vol. 32, Issue 2, pp. 175-86, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Barda-Saad, Shirasu, Pauker et al.: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).