Aryl Hydrocarbon Receptor (AHR) antibody

Antigen: Aryl Hydrocarbon Receptor (AHR)

Synonyms: bHLHe76, AHR, Ah, In, Ahh, Ahre, ahr1, ahr, Ahr

Clonality: Monoclonal (3B12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394219

Additional Information

Alternative name: AHR

Gene ID: 196

UniProt: NP_001612

Immunogen: AHR (NP_001612, 721 a.a. ~ 821 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3B12

Description: Other names: aryl hydrocarbon receptor

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AHR (NP_001612, 721 a.a. ~ 821 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 96147 DA

Comments:

Background: This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to AHR (AT1073a) on HeLa cell . [antibody concentration 10 ug/ml] Western blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # AT1073a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Aryl Hydrocarbon Receptor (AHR) antibody (Image 2)

Publications

Product: Rowlands, Staskal, Gollapudi et al.: "The human AHR: identification of single nucleotide polymorphisms from six ethnic populations." in: Pharmacogenetics and genomics, Vol. 20, Issue 5, pp. 283-90, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Tan, Wang, Yang et al.: "Aryl hydrocarbon receptor is a transcriptional activator of the human breast cancer resistance protein (BCRP/ABCG2)." in: Molecular pharmacology, Vol. 78, Issue 2, pp. 175-85, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Gandhi, Kumar, Burns et al.: "Activation of the aryl hydrocarbon receptor induces human type 1 regulatory T cell-like and Foxp3(+) regulatory T cells." in: Nature immunology, Vol. 11, Issue 9, pp. 846-53, 2010 (PubMed).