Salvador Homolog 1 (Drosophila) (SAV1) antibody

Antigen: Salvador Homolog 1 (Drosophila) (SAV1)

Synonyms: SAV, WW45, WWP4

Clonality: Monoclonal (3B2)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394223

Additional Information

Alternative name: SAV1

Gene ID: 60485

UniProt: NP_068590

Immunogen: SAV1 (NP_068590, 300 a.a. ~ 384 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3B2

Description: Other names: SAV, WW45, WWP4, salvador salvador homolog 1 (Drosophila)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: SAV1 (NP_068590, 300 a.a. ~ 384 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 44634 DA

Comments:

Background: WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a coiled-coil region. It is ubiquitously expressed in adult tissues. The encoded protein is 94% identical to the mouse protein at the amino acid level.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to SAV1 (AT3774a) on HeLa cell . [antibody concentration 60 ug/ml] SAV1 monoclonal antibody (M02), clone 3B2 Western Blot analysis of SAV1 expression in Hela NE ( Cat # L013V3 ).

anti-Salvador Homolog 1 (Drosophila) (SAV1) antibody (Image 2)

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Chan, Nousiainen, Chalamalasetty et al.: "The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1." in: Oncogene, Vol. 24, Issue 12, pp. 2076-86, 2005 (PubMed).

Benzinger, Muster, Koch et al.: "Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 6, pp. 785-95, 2005 (PubMed).

Callus, Verhagen, Vaux: "Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation." in: The FEBS journal, Vol. 273, Issue 18, pp. 4264-76, 2006 (PubMed).

Luo, Li, Yan et al.: "The human WW45 protein enhances MST1-mediated apoptosis in vivo." in: International journal of molecular medicine, Vol. 23, Issue 3, pp. 357-62, 2009 (PubMed).