NK3 Homeobox 1 (NKX3-1) antibody
| Antigen | NK3 Homeobox 1 (NKX3-1) |
| Synonyms | NKX3, BAPX2, NKX3A, NKX3.1, Bax, Nkx-3.1, bagpipe, MGC124859, nkx3-1, MGC53041, MGC97890, NKX3-1, Xbap, nkx3a, nkx3.1 |
| Clonality | Monoclonal (3C1) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB) |
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5 references available |
| Certificates | ISO 9001:2008 |
| Catalog no. | ABIN394230 |
| Quantity | 0.1mg |
| Price | 450.00 $ Plus shipping costs $45.00 |
| Shipping to |
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| Availability | Will be delivered in 2 to 3 Business Days |
Additional Information
| Alternative name | NKX3-1 |
| Gene ID | 4824 |
| UniProt | NP_006158 |
| Immunogen | NKX3-1 (NP_006158, 100 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K |
| Isotype | IgG2b kappa |
| Clone | 3C1 |
| Description | Other names: NKX3.1, NKX3A NK3 transcription factor related, locus 1 (Drosophila) |
| Characteristics | Purified Mouse Monoclonal Antibody (Mab) |
| Specificity | NKX3-1 (NP_006158, 100 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Molecular Weight | 26350 DA |
| Comments |
Background: The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia. |
Application Details
| Application Notes | ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 |
| Purification | Purified |
| Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles |
| Research Area | Cancer, Transcription Factors, Signaling, Cell Structure |
| Restrictions | For Research Use only |
Images
| Detection limit for recombinant GST tagged NKX3-1 is approximately 1ng/ml as a capture antibody. |
Publications
| Product |
Thomas, Preece, Bentel: "Androgen regulation of the prostatic tumour suppressor NKX3.1 is mediated by its 3' untranslated region." in: The Biochemical journal, Vol. 425, Issue 3, pp. 575-83, 2010 (PubMed).
Bowen, Gelmann: "NKX3.1 activates cellular response to DNA damage." in: Cancer research, Vol. 70, Issue 8, pp. 3089-97, 2010 (PubMed). Kunderfranco, Mello-Grand, Cangemi et al.: "ETS transcription factors control transcription of EZH2 and epigenetic silencing of the tumor suppressor gene Nkx3.1 in prostate cancer." in: PLoS ONE, Vol. 5, Issue 5, pp. e10547, 2010 (PubMed). Kim, Cheng, Hsu et al.: "Prostate cancer risk-associated variants reported from genome-wide association studies: meta-analysis and their contribution to genetic Variation." in: The Prostate, Vol. 70, Issue 16, pp. 1729-38, 2010 (PubMed). Gurel, Ali, Montgomery et al.: "NKX3.1 as a marker of prostatic origin in metastatic tumors." in: The American journal of surgical pathology, Vol. 34, Issue 8, pp. 1097-105, 2010 (PubMed). |
Alternatives
Alternatives for antigen "NK3 Homeobox 1 (NKX3-1)", type "Antibodies"
| Hosts | Rabbit (29), Mouse (13) |
| Reactivities | Human (39), Mouse (Murine) (10), Rat (Rattus) (7), Cow (Bovine) (2), Cat (Feline) (1), Chicken (1), Dog (Canine) (1), Fruit Fly (Drosophila melanogaster) (1), Xenopus laevis (1) |
| Applications | Western Blotting (WB) (34), ELISA (22), Immunohistochemistry (IHC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Flow Cytometry (FACS) (8), Immunofluorescence (IF) (2), Dot Blot (Dot) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunoprecipitation (IP) (1) |
| Epitopes | N-Term (6), AA 21-37 (2), Internal Region (2), C-Term (1), C-Term, AA 21-37 (1), C-Term,AA 158-187 (1), C-Term,AA 21-37 (1), Center (1), Internal Region,AA 124-154 (1), Internal Region,AA 58-86 (1), Middle Region,Internal Region (1) |
