SRY (Sex Determining Region Y)-Box 9 (SOX9) antibody

Antigen: SRY (Sex Determining Region Y)-Box 9 (SOX9)

Synonyms: CMD1, SRA1, CMPD1, LOC100227849

Clonality: Monoclonal (3C10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394231

Additional Information

Alternative name: SOX9

Gene ID: 6662

UniProt: NP_000337

Immunogen: SOX9 (NP_000337, 400 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3C10

Description: Other names: CMD1, CMPD1, SRA1SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: SOX9 (NP_000337, 400 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 56137 DA

Comments:

Background: The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 0.5mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to SOX9 (AT4001a) on HepG2 cell . [antibody concentration 10 ug/ml] SOX9 monoclonal antibody (M02), clone 3C10 Western Blot analysis of SOX9 expression in HepG2 ( Cat # L019V1 ).

anti-SRY (Sex Determining Region Y)-Box 9 (SOX9) antibody (Image 2)

Publications

Product: Rao, Fuller, Prieto: "Expression of Sox-9 in metastatic melanoma--a potential diagnostic pitfall." in: The American Journal of dermatopathology, Vol. 32, Issue 3, pp. 262-6, 2010 (PubMed).

Thomsen, Ambroisine, Wynn et al.: "SOX9 elevation in the prostate promotes proliferation and cooperates with PTEN loss to drive tumor formation." in: Cancer research, Vol. 70, Issue 3, pp. 979-87, 2010 (PubMed).

Masuda, Esumi: "SOX9, through interaction with microphthalmia-associated transcription factor (MITF) and OTX2, regulates BEST1 expression in the retinal pigment epithelium." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 26933-44, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).