RECQL2 (ARABIDOPSIS RECQ HELICASE L2); 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2) antibody

Antigen: RECQL2 (ARABIDOPSIS RECQ HELICASE L2); 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2)

Synonyms: RECQ3, RECQL2, RECQL3, DKFZp686C2056, ARABIDOPSIS RECQ HELICASE L2, ARABIDOPSIS THALIANA RECQ 2, ATRECQ2, T19E23.16, T19E23_16, xBLM, recq2, recql2, recql3

Clonality: Monoclonal (3C11)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394233

Additional Information

Alternative name: WRN

Gene ID: 7486

UniProt: NP_000544

Immunogen: WRN (NP_000544, 1322 a.a. ~ 1433 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3C11

Description: Other names: RECQ3, RECQL2, RECQL3 Werner syndrome

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: WRN (NP_000544, 1322 a.a. ~ 1433 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 162495 DA

Comments:

Background: This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to WRN (AT4544a) on HeLa cell . [antibody concentration 10 ug/ml] WRN monoclonal antibody (M09), clone 3C11 Western Blot analysis of WRN expression in Hela NE ( Cat # L013V3 ).

anti-RECQL2 (ARABIDOPSIS RECQ HELICASE L2); 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2) antibody (Image 2)

Publications

Product: Olson, Wang, Pankratz et al.: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010 (PubMed).

Perry, Asaithamby, Barnebey et al.: "Identification of a coiled coil in werner syndrome protein that facilitates multimerization and promotes exonuclease processivity." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25699-707, 2010 (PubMed).

Briggs, Goldstein, McCauley et al.: "Variation within DNA repair pathway genes and risk of multiple sclerosis." in: American journal of epidemiology, Vol. 172, Issue 2, pp. 217-24, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Ehrenberg, Dratviman-Storobinsky, Avraham-Lubin et al.: "Lack of association of the WRN C1367T polymorphism with senile cataract in the Israeli population." in: Molecular vision, Vol. 16, pp. 1771-5, 2010 (PubMed).