You are viewing an incomplete version of our website. Please click to reload the website as full version.

RECQL2 (ARABIDOPSIS RECQ HELICASE L2), 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2) (AA 1322-1433) antibody

Details for Product No. ABIN394233, Supplier: Log in to see
Antigen
  • AI846146
  • recq2
  • RECQ3
  • recql2
  • RECQL2
  • RECQL3
  • recql3
  • xBLM
Alternatives
anti-Human RECQL2 (ARABIDOPSIS RECQ HELICASE L2), 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding antibody for Western Blotting
Epitope
AA 1322-1433
16
13
12
10
9
3
3
3
2
2
2
2
1
Reactivity
Human
51
2
1
1
Host
Mouse
33
20
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
47
30
17
10
9
6
4
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen WRN (NP_000544, 1322 a.a. ~ 1433 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3C11
Isotype IgG2a kappa
Specificity WRN (NP_000544, 1322 a.a. ~ 1433 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name WRN (RECQL2 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 162495 DA
Gene ID 12406
NCBI Accession NP_000544
Research Area Transcription Factors, Signaling, Metabolism, Cell Structure
Pathways DNA Damage Repair
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-RECQL2 (ARABIDOPSIS RECQ HELICASE L2), 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2) (AA 1322-1433) antibody (ABIN394233) Immunofluorescence of monoclonal antibody to WRN on HeLa cell (antibody concentration...
Immunohistochemistry (IHC) image for anti-RECQL2 (ARABIDOPSIS RECQ HELICASE L2), 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2) (AA 1322-1433) antibody (ABIN394233) anti-RECQL2 (ARABIDOPSIS RECQ HELICASE L2), 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding (RECQL2) (AA 1322-1433) antibody (Image 2)
Background publications Ehrenberg, Dratviman-Storobinsky, Avraham-Lubin, Goldenberg-Cohen: "Lack of association of the WRN C1367T polymorphism with senile cataract in the Israeli population." in: Molecular vision, Vol. 16, pp. 1771-5, 2010

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Briggs, Goldstein, McCauley, Zuvich, De Jager, Rioux, Ivinson, Compston, Hafler, Hauser, Oksenberg, Sawcer, Pericak-Vance, Haines, Barcellos: "Variation within DNA repair pathway genes and risk of multiple sclerosis." in: American journal of epidemiology, Vol. 172, Issue 2, pp. 217-24, 2010

Perry, Asaithamby, Barnebey, Kiamanesch, Chen, Han, Tainer, Yannone: "Identification of a coiled coil in werner syndrome protein that facilitates multimerization and promotes exonuclease processivity." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25699-707, 2010

Olson, Wang, Pankratz, Fredericksen, Vachon, Vierkant, Cerhan, Couch: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010