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Id2 (AA 1-135) antibody

Details for Product No. ABIN394238, Supplier: Log in to see
Antigen
  • Ac2-300
  • AI255428
  • bHLHb26
  • C78922
  • GIG8
  • id2-A
  • ID2A
  • ID2H
  • Idb2
  • XId2
Epitope
AA 1-135
15
13
5
5
2
1
1
1
1
1
1
1
Reactivity
Human
92
30
29
3
3
2
2
2
1
Host
Mouse
59
32
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
64
32
26
12
11
11
9
8
4
2
1
Supplier
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Immunogen ID2 (AAH30639, 1 a.a. ~ 135 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3C3
Isotype IgG2a kappa
Specificity ID2 (AAH30639, 1 a.a. ~ 135 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 14917 DA
Gene ID 12406
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Id2 (AA 1-135) antibody (ABIN394238) Western blot analysis of ID2 over-expressed 293 cell line, cotransfected with ID2 Val...
Immunohistochemistry (IHC) image for anti-Id2 (AA 1-135) antibody (ABIN394238) anti-Id2 (AA 1-135) antibody (Image 2)
Background publications Johnatty, Beesley, Chen, Macgregor, Duffy, Spurdle, deFazio, Gava, Webb, Rossing, Doherty, Goodman, Lurie, Thompson, Wilkens, Ness, Moysich, Chang-Claude, Wang-Gohrke, Cramer, Terry, Hankinson, Tworoger, Garcia-Closas, Yang, Lissowska, Chanock, Pharoah, S: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).

Nagel, Venturini, Marquez, Meyer, Kaufmann, Scherr, MacLeod, Drexler: "Polycomb repressor complex 2 regulates HOXA9 and HOXA10, activating ID2 in NK/T-cell lines." in: Molecular cancer, Vol. 9, pp. 151, 2010 (PubMed).

Schotte, Dontje, Nagasawa, Yasuda, Bakker, Spits, Blom: "Synergy between IL-15 and Id2 promotes the expansion of human NK progenitor cells, which can be counteracted by the E protein HEB required to drive T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 12, pp. 6670-9, 2010 (PubMed).

Coma, Amin, Shimizu, Lasorella, Iavarone, Klagsbrun: "Id2 promotes tumor cell migration and invasion through transcriptional repression of semaphorin 3F." in: Cancer research, Vol. 70, Issue 9, pp. 3823-32, 2010 (PubMed).

Kurowska-Stolarska, Distler, Jüngel, Rudnicka, Neumann, Pap, Wenger, Michel, Müller-Ladner, Gay, Maslinski, Gay, Distler: "Inhibitor of DNA binding/differentiation 2 induced by hypoxia promotes synovial fibroblast-dependent osteoclastogenesis." in: Arthritis and rheumatism, Vol. 60, Issue 12, pp. 3663-75, 2009 (PubMed).