Id2 antibody

Antigen: Id2

Clonality: Monoclonal (3C3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394238

Additional Information

Gene ID: 3398

Immunogen: ID2 (AAH30639, 1 a.a. ~ 135 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3C3

Description: Other names: GIG8, ID2A, ID2H, MGC26389 inhibitor of DNA binding 2, dominant negative helix-loop-helix protein

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ID2 (AAH30639, 1 a.a. ~ 135 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 14917 DA

Comments:

Background: The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ID2 over-expressed 293 cell line, cotransfected with ID2 Validated Chimera RNAi ( Cat # H00003398-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID2 monoclonal antibody (M01), clone 3C3 (Cat # AT2475a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of ID2 expression in transfected 293T cell line by ID2 monoclonal antibody (M01), clone 3C3. Lane 1: ID2 transfected lysate(14.9 KDa). Lane 2: Non-transfected lysate.

anti-Id2 antibody (Image 2)

Publications

Product: Kurowska-Stolarska, Distler, Jüngel et al.: "Inhibitor of DNA binding/differentiation 2 induced by hypoxia promotes synovial fibroblast-dependent osteoclastogenesis." in: Arthritis and rheumatism, Vol. 60, Issue 12, pp. 3663-75, 2009 (PubMed).

Coma, Amin, Shimizu et al.: "Id2 promotes tumor cell migration and invasion through transcriptional repression of semaphorin 3F." in: Cancer research, Vol. 70, Issue 9, pp. 3823-32, 2010 (PubMed).

Schotte, Dontje, Nagasawa et al.: "Synergy between IL-15 and Id2 promotes the expansion of human NK progenitor cells, which can be counteracted by the E protein HEB required to drive T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 12, pp. 6670-9, 2010 (PubMed).

Nagel, Venturini, Marquez et al.: "Polycomb repressor complex 2 regulates HOXA9 and HOXA10, activating ID2 in NK/T-cell lines." in: Molecular cancer, Vol. 9, pp. 151, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).