Id2 antibody

Details for Product No. ABIN394238
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Antigen
Synonyms Idb2, C78922, bHLHb26, AI255428, Ac2-300, GIG8, ID2A, ID2H, XId2, id2-A
Reactivity
Human
(54), (20), (19), (13), (13), (13), (12)
Host
Mouse
(34), (21), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(31), (17), (15), (10), (10), (4), (4), (2), (2), (2), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN394238
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen ID2 (AAH30639, 1 a.a. ~ 135 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3C3
Isotype IgG2a kappa
Specificity ID2 (AAH30639, 1 a.a. ~ 135 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 14917 DA
Gene ID 12406
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Id2 antibody Western blot analysis of ID2 over-expressed 293 cell line, cotransfected with ID2 Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of ID2 expression in transfected 293T cell line by ID2 monoclonal antibody (M01), clone 3C3. Lane 1: ID2 transfected lysate(14.9 KDa). Lane 2: Non-transfected lysate.
anti-Id2 antibody (2) anti-Id2 antibody (Image 2)
Background publications Kurowska-Stolarska, Distler, Jüngel et al.: "Inhibitor of DNA binding/differentiation 2 induced by hypoxia promotes synovial fibroblast-dependent osteoclastogenesis." in: Arthritis and rheumatism, Vol. 60, Issue 12, pp. 3663-75, 2009 (PubMed).

Coma, Amin, Shimizu et al.: "Id2 promotes tumor cell migration and invasion through transcriptional repression of semaphorin 3F." in: Cancer research, Vol. 70, Issue 9, pp. 3823-32, 2010 (PubMed).

Schotte, Dontje, Nagasawa et al.: "Synergy between IL-15 and Id2 promotes the expansion of human NK progenitor cells, which can be counteracted by the E protein HEB required to drive T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 12, pp. 6670-9, 2010 (PubMed).

Nagel, Venturini, Marquez et al.: "Polycomb repressor complex 2 regulates HOXA9 and HOXA10, activating ID2 in NK/T-cell lines." in: Molecular cancer, Vol. 9, pp. 151, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).

Hosts (34), (21), (1)
Reactivities (54), (20), (19), (13), (13), (13), (12)
Applications (31), (17), (15), (10), (10), (4), (4), (2), (2), (2), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (5), (2), (1), (1), (1), (1)
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