Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 1-110) antibody

Details for Product No. ABIN394250
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Antigen
Synonyms ARNT, zgc:136664, arnt1, hif1b, tango, xArnt, hif1beta, hif-1beta, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2, D3Ertd557e, Drnt, ESTM42, Hif1b, W08714, mKIAA4051, Arnt1
Epitope
AA 1-110
(12), (9), (5), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(83), (40), (35), (21), (13), (12), (9), (9), (1), (1)
Host
Mouse
(51), (32), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(71), (28), (24), (20), (12), (10), (9), (5), (4), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN394250
450.00 $
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Immunogen ARNT (AAH60838, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D10
Isotype IgG2a kappa
Specificity ARNT (AAH60838, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ARNT
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 86636 DA
Gene ID 12406
Research Area Transcription Factors, Signaling, Metabolism, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1,12)(q21,p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 1-110) antibody Immunofluorescence of monoclonal antibody to ARNT on HeLa cell (antibody concentration 10 µg/mL). Western blot analysis of ARNT over-expressed 293 cell line, cotransfected with ARNT Validated Chimera RNAi used as specificity and loading control.
anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 1-110) antibody (2) anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 1-110) antibody (Image 2)
Background publications Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Cao, Deng, Wan et al.: "MicroRNA-101 negatively regulates Ezh2 and its expression is modulated by androgen receptor and HIF-1alpha/HIF-1beta." in: Molecular cancer, Vol. 9, pp. 108, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Validation Images
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