Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody

Antigen: Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)

Synonyms: HIF1B, TANGO, bHLHe2, HIF1BETA, HIF-1beta, Drnt, Hif1b, ESTM42, W08714, KIAA4051, mKIAA4051, D3Ertd557e, Arnt1, ARNT, MGC136664, zgc:136664, arnt1, hif1b, tango, xArnt, hif1beta, hif-1beta, AHA-1

Clonality: Monoclonal (3D10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394250

Additional Information

Alternative name: ARNT

Gene ID: 405

Immunogen: ARNT (AAH60838, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3D10

Description: Other names: HIF1beta, HIF1B, HIF1BETA, TANGO aryl hydrocarbon receptor nuclear translocator

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ARNT (AAH60838, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 86636 DA

Comments:

Background: The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1,12)(q21,p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to ARNT (AT1199a) on HeLa cell . [antibody concentration 10 ug/ml] Western blot analysis of ARNT over-expressed 293 cell line, cotransfected with ARNT Validated Chimera RNAi ( Cat # H00000405-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ARNT monoclonal antibody (M01), clone 3D10 (Cat # AT1199a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody (Image 2)

Publications

Product: Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Cao, Deng, Wan et al.: "MicroRNA-101 negatively regulates Ezh2 and its expression is modulated by androgen receptor and HIF-1alpha/HIF-1beta." in: Molecular cancer, Vol. 9, pp. 108, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).