DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6)

Synonyms: P54, RCK, HLR2, FLJ36338, DDX6, RGD1564560, si:ch211-147p17.1, p54, rck, P54H, Xp54, hlr2, me31b, MGC145663

Clonality: Monoclonal (3D2)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394255

Additional Information

Alternative name: DDX6

Gene ID: 1656

Immunogen: DDX6 (AAH65007, 1 a.a. ~ 484 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3D2

Description: Other names: HLR2, P54, RCKDEAD (Asp-Glu-Ala-Asp) box polypeptide 6

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DDX6 (AAH65007, 1 a.a. ~ 484 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 54417 DA

Comments:

Background: This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2. Lane 1: DDX6 transfected lysate(53.2 KDa). Lane 2: Non-transfected lysate. Western blot analysis of DDX6 over-expressed 293 cell line, cotransfected with DDX6 Validated Chimera RNAi ( Cat # H00001656-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DDX6 monoclonal antibody (M01), clone 3D2 (Cat # AT1743a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) antibody (Image 2)

Publications

Product: Tritschler, Braun, Eulalio et al.: "Structural basis for the mutually exclusive anchoring of P body components EDC3 and Tral to the DEAD box protein DDX6/Me31B." in: Molecular cell, Vol. 33, Issue 5, pp. 661-8, 2009 (PubMed).

Minshall, Kress, Weil et al.: "Role of p54 RNA helicase activity and its C-terminal domain in translational repression, P-body localization and assembly." in: Molecular biology of the cell, Vol. 20, Issue 9, pp. 2464-72, 2009 (PubMed).

Han, Zheng, Cui et al.: "Genome-wide association study in a Chinese Han population identifies nine new susceptibility loci for systemic lupus erythematosus." in: Nature genetics, Vol. 41, Issue 11, pp. 1234-7, 2009 (PubMed).

Lind, Macgregor, Vink et al.: "A genomewide association study of nicotine and alcohol dependence in Australian and Dutch populations." in: Twin research and human genetics : the official journal of the International Society for Twin Studies, Vol. 13, Issue 1, pp. 10-29, 2010 (PubMed).

Jangra, Yi, Lemon: "DDX6 (Rck/p54) is required for efficient hepatitis C virus replication but not for internal ribosome entry site-directed translation." in: Journal of virology, Vol. 84, Issue 13, pp. 6810-24, 2010 (PubMed).