ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2) (AA 188-254) antibody

Details for Product No. ABIN394259
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Antigen
Synonyms ATP6C2, VMA5, 1110038G14Rik
Epitope
AA 188-254
(15), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(37), (3), (2)
Host
Mouse
(34), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(36), (24), (3), (3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394259
450.00 $
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Immunogen ATP6V1C2 (NP_653184, 188 a.a. ~ 254 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D5
Isotype IgG2b kappa
Specificity ATP6V1C2 (NP_653184, 188 a.a. ~ 254 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ATP6V1C2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 48759 DA
Gene ID 12406
NCBI Accession NP_653184
Research Area Cell Structure, Transcription Factors, Signaling
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2) (AA 188-254) antibody ATP6V1C2 monoclonal antibody (M01), clone 3D5 Western Blot analysis of ATP6V1C2 expression in HeLa. Detection limit for recombinant GST tagged ATP6V1C2 is approximately 0.03 ng/mL as a capture antibody.
anti-ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2) (AA 188-254) antibody (2) anti-ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2) (AA 188-254) antibody (Image 2)
Background publications Morel: "Neurotransmitter release: the dark side of the vacuolar-H+ATPase." in: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 95, Issue 7, pp. 453-7, 2003 (PubMed).

Fu, Wang, Yang et al.: "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes." in: Genomics, Vol. 84, Issue 1, pp. 205-10, 2004 (PubMed).

Grüber: "Structural features and nucleotide-binding capability of the C subunit are integral to the regulation of the eukaryotic V1Vo ATPases." in: Biochemical Society transactions, Vol. 33, Issue Pt 4, pp. 883-5, 2005 (PubMed).

Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

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