Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN394259

ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms VMA5, ATP6C2, 1110038G14Rik
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN394259
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Immunogen ATP6V1C2 (NP_653184, 188 a.a. ~ 254 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D5
Isotype IgG2b kappa
Specificity ATP6V1C2 (NP_653184, 188 a.a. ~ 254 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ATP6V1C2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 48759 DA
Gene ID 12406
UniProt NP_653184
Research Area Cell Structure, Transcription Factors, Signaling
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Morel: "Neurotransmitter release: the dark side of the vacuolar-H+ATPase." in: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 95, Issue 7, pp. 453-7, 2003 (PubMed).

Fu, Wang, Yang et al.: "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes." in: Genomics, Vol. 84, Issue 1, pp. 205-10, 2004 (PubMed).

Grüber: "Structural features and nucleotide-binding capability of the C subunit are integral to the regulation of the eukaryotic V1Vo ATPases." in: Biochemical Society transactions, Vol. 33, Issue Pt 4, pp. 883-5, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Alternatives for antigen "ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2)", type "Antibodies"
Hosts (21), (3)
Reactivities (24), (2), (2)
Applications (24), (12), (3), (2), (1)
Epitopes (8), (3), (2), (2), (2), (2), (1), (1), (1)
Beacon