ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2) antibody

Antigen: ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2)

Synonyms: VMA5, ATP6C2

Clonality: Monoclonal (3D5)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394259

Additional Information

Alternative name: ATP6V1C2

Gene ID: 245973

UniProt: NP_653184

Immunogen: ATP6V1C2 (NP_653184, 188 a.a. ~ 254 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3D5

Description: Other names: ATP6C2, VMA5 ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C isoform 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ATP6V1C2 (NP_653184, 188 a.a. ~ 254 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 48759 DA

Comments:

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

ATP6V1C2 monoclonal antibody (M01), clone 3D5 Western Blot analysis of ATP6V1C2 expression in HeLa ( Cat # L013V1 ). Detection limit for recombinant GST tagged ATP6V1C2 is approximately 0.03ng/ml as a capture antibody.

anti-ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C2 (ATP6V1C2) antibody (Image 2)

Publications

Product: Morel: "Neurotransmitter release: the dark side of the vacuolar-H+ATPase." in: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 95, Issue 7, pp. 453-7, 2003 (PubMed).

Fu, Wang, Yang et al.: "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes." in: Genomics, Vol. 84, Issue 1, pp. 205-10, 2004 (PubMed).

Grüber: "Structural features and nucleotide-binding capability of the C subunit are integral to the regulation of the eukaryotic V1Vo ATPases." in: Biochemical Society transactions, Vol. 33, Issue Pt 4, pp. 883-5, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).