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Details for Product No. ABIN394269

E2F Transcription Factor 8 (E2F8) antibody

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Synonyms E2F-8, AA410048, 4432406C08Rik, RGD1308091, MGC130750, E2F8
»Alternatives Human
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
»Alternatives Un-conjugated
»Alternatives ELISA, Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN394269
Quantity 0.1 mg
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen E2F8 (AAH28244, 1 a.a. ~ 432 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3E9-2F5
Isotype IgG1 kappa
Specificity E2F8 (AAH28244, 1 a.a. ~ 432 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name E2F8
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 94166 DA
Gene ID 12406
Research Area Cell Cycle, Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Background: E2F transcription factors, such as E2F8, are essential for orchestrating expression of genes required for cell cycle progression and proliferation (Christensen et al., 2005 [PubMed 16179649]).

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Maiti, Li, de Bruin et al.: "Cloning and characterization of mouse E2F8, a novel mammalian E2F family member capable of blocking cellular proliferation." in: The Journal of biological chemistry, Vol. 280, Issue 18, pp. 18211-20, 2005 (PubMed).

Logan, Graham, Zhao et al.: "E2F-8: an E2F family member with a similar organization of DNA-binding domains to E2F-7." in: Oncogene, Vol. 24, Issue 31, pp. 5000-4, 2005 (PubMed).

Christensen, Cloos, Toftegaard et al.: "Characterization of E2F8, a novel E2F-like cell-cycle regulated repressor of E2F-activated transcription." in: Nucleic acids research, Vol. 33, Issue 17, pp. 5458-70, 2005 (PubMed).

Zalmas, Zhao, Graham et al.: "DNA-damage response control of E2F7 and E2F8." in: EMBO reports, Vol. 9, Issue 3, pp. 252-9, 2008 (PubMed).

Deng, Wang, Zong et al.: "E2F8 contributes to human hepatocellular carcinoma via regulating cell proliferation." in: Cancer research, Vol. 70, Issue 2, pp. 782-91, 2010 (PubMed).

Alternatives for antigen "E2F Transcription Factor 8 (E2F8)", type "Antibodies"
Hosts (23), (3), (1)
Reactivities (27), (18), (18), (12), (12), (1), (1)
Applications (15), (10), (10), (3), (3), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (1), (1), (1)