Poly (ADP-Ribose) Polymerase 1 (PARP1) antibody

Antigen: Poly (ADP-Ribose) Polymerase 1 (PARP1)

Synonyms: PARP, PPOL, ADPRT, ADPRT1, PARP-1, pADPRT-1, RT6, ART2, CD296, MGC133217, Yac-1, Adprt, Parp-1, MGC93658, ART1, ART7.2, ART7.1

Clonality: Monoclonal (3G4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394297

Additional Information

Alternative name: PARP1

Gene ID: 142

Immunogen: PARP1 (AAH37545, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3G4

Description: Other names: ADPRT, ADPRT1, PARP, PARP1, PPOL, pADPRT1 poly (ADP-ribose) polymerase family, member 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: PARP1 (AAH37545, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 113084 DA

Comments:

Background: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to PARP1 (AT3183a) on HeLa cell . [antibody concentration 10 ug/ml] PARP1 monoclonal antibody (M01), clone 3G4 Western Blot analysis of PARP1 expression in HeLa NE ( Cat # L013V3 ).

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) antibody (Image 2)

Publications

Product: Arora, Lindgren, Basu et al.: "Polymorphisms in the base excision repair pathway and graft-versus-host disease." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 24, Issue 8, pp. 1470-5, 2010 (PubMed).

Krishnakumar, Kraus: "The PARP side of the nucleus: molecular actions, physiological outcomes, and clinical targets." in: Molecular cell, Vol. 39, Issue 1, pp. 8-24, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Ho-Pun-Cheung, Assenat, Bascoul-Mollevi et al.: "A large-scale candidate gene approach identifies SNPs in SOD2 and IL13 as predictive markers of response to preoperative chemoradiation in rectal cancer." in: The pharmacogenomics journal, Vol. 11, Issue 6, pp. 437-43, 2011 (PubMed).

Lee, Bang, Park et al.: "Lack of association between poly(ADP-ribose) polymerase (PARP) polymorphisms and rheumatoid arthritis in a Korean population." in: Rheumatology international, Vol. 32, Issue 1, pp. 91-6, 2012 (PubMed).