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TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7) (AA 130-224) antibody

Details for Product No. ABIN394299
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Antigen
Synonyms CG2670, Dmel\\CG2670, E, TAF, TAF55, TAF7, TAF[[II]], TAF[[II]]55, TFIID, Taf55, anon-84Ec, dTAF[[II]]55, AO090012000400, CHUNP6888, 55kDa, AI607308, BB007175, TAFII55, Taf2f, TAF2F, taf2f
Epitope
AA 130-224
(9), (9), (8), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(32), (4), (4), (2), (1), (1), (1)
Host
Mouse
(20), (11), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(31), (19), (12), (6), (4), (4), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394299
450.00 $
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Immunogen TAF7 (AAH32737, 130 a.a. ~ 224 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G6
Isotype IgG2a kappa
Specificity TAF7 (AAH32737, 130 a.a. ~ 224 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TAF7
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 40259 DA
Gene ID 12406
Research Area Cell Structure, Transcription Factors, Signaling
Application Notes Western blot = 1:500-1000
Comment

Background: The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7) (AA 130-224) antibody TAF7 monoclonal antibody (M02), clone 3G6 Western Blot analysis of TAF7 expression in Hela NE. Detection limit for recombinant GST tagged TAF7 is approximately 0.1 ng/mL as a capture antibody.
anti-TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7) (AA 130-224) antibody (2) anti-TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7) (AA 130-224) antibody (Image 2)
Background publications Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Hartman, Walters, Hentosh: "Presence of the anti-leukemic nucleotide analog, 2-chloro-2'-deoxyadenosine-5'-monophosphate, in a promoter sequence alters DNA binding of TATA-binding protein (TBP)." in: Archives of biochemistry and biophysics, Vol. 459, Issue 2, pp. 223-32, 2007 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Gegonne, Weissman, Lu et al.: "TFIID component TAF7 functionally interacts with both TFIIH and P-TEFb." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 14, pp. 5367-72, 2008 (PubMed).

Validation Images
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