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Zinc Finger Protein 163 (ZNF163) (AA 1-92) antibody

Details for Product No. ABIN394301, Supplier: Log in to see
Antigen
  • AW495828
  • Gfi-1
  • GFI-1
  • GFI1A
  • Pal-1
  • Pal1
  • SCN2
  • ZNF163
Epitope
AA 1-92
8
6
6
5
3
2
1
1
1
1
1
Reactivity
Human
57
13
12
4
4
3
2
Host
Mouse
32
27
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
54
26
13
12
6
5
1
1
Supplier
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Immunogen GFI1 (NP_005254, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G8
Isotype IgG1 kappa
Specificity GFI1 (NP_005254, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name GFI1 (ZNF163 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 37492 DA
Gene ID 12406
NCBI Accession NP_005254
Research Area Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Zinc Finger Protein 163 (ZNF163) (AA 1-92) antibody (ABIN394301) Immunofluorescence of monoclonal antibody to GFI1 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-Zinc Finger Protein 163 (ZNF163) (AA 1-92) antibody (ABIN394301) anti-Zinc Finger Protein 163 (ZNF163) (AA 1-92) antibody (Image 2)
Background publications Alcina, Fernández, Gonzalez, Catalá-Rabasa, Fedetz, Ndagire, Leyva, Guerrero, Arnal, Delgado, Lucas, Izquierdo, Matesanz: "Tag-SNP analysis of the GFI1-EVI5-RPL5-FAM69 risk locus for multiple sclerosis." in: European journal of human genetics : EJHG, Vol. 18, Issue 7, pp. 827-31, 2010 (PubMed).

Khandanpour, Thiede, Valk, Sharif-Askari, Nückel, Lohmann, Horsthemke, Siffert, Neubauer, Grzeschik, Bloomfield, Marcucci, Maharry, Slovak, van der Reijden, Jansen, Schackert, Afshar, Schnittger, Peeters, Kroschinsky, Ehninger, Lowenberg, Dührsen, Möröy: "A variant allele of Growth Factor Independence 1 (GFI1) is associated with acute myeloid leukemia." in: Blood, Vol. 115, Issue 12, pp. 2462-72, 2010 (PubMed).

Huang, Hu, Chang, Ou, Zhou, Zhang: "The growth factor independence-1 (Gfi1) is overexpressed in chronic myelogenous leukemia." in: Acta haematologica, Vol. 123, Issue 1, pp. 1-5, 2009 (PubMed).

Salipante, Rojas, Korkmaz, Duan, Wechsler, Benson, Person, Grimes, Horwitz: "Contributions to neutropenia from PFAAP5 (N4BP2L2), a novel protein mediating transcriptional repressor cooperation between Gfi1 and neutrophil elastase." in: Molecular and cellular biology, Vol. 29, Issue 16, pp. 4394-405, 2009 (PubMed).

Basu, Liu, Qiu, Dong: "Gfi-1 represses CDKN2B encoding p15INK4B through interaction with Miz-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 5, pp. 1433-8, 2009 (PubMed).