TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kDa (TAF11) antibody

Antigen: TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kDa (TAF11)

Synonyms: TAF2I, PRO2134, TAFII28, MGC:15243

Clonality: Monoclonal (3H5)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394309

Additional Information

Alternative name: TAF11

Gene ID: 6882

UniProt: NP_005634

Immunogen: TAF11 (NP_005634, 158 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3H5

Description: Other names: MGC:15243, PRO2134, TAF2I, TAFII28 TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: TAF11 (NP_005634, 158 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 23307 DA

Comments:

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to TAF11 (AT4139a) on HeLa cell . [antibody concentration 10 ug/ml] TAF11 monoclonal antibody (M03), clone 3H5 Western Blot analysis of TAF11 expression in Hela NE ( Cat # L013V3 ).

anti-TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kDa (TAF11) antibody (Image 2)

Publications

Product: Mengus, Gangloff, Carré et al.: "The human transcription factor IID subunit human TATA-binding protein-associated factor 28 interacts in a ligand-reversible manner with the vitamin D(3) and thyroid hormone receptors." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10064-71, 2000 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Mungall, Palmer, Sims et al.: "The DNA sequence and analysis of human chromosome 6." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003 (PubMed).

Guermah, Ge, Chiang et al.: "The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis." in: Molecular cell, Vol. 12, Issue 4, pp. 991-1001, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).