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SET Domain, Bifurcated 1 (SETDB1) (AA 2-101) antibody

Details for Product No. ABIN394317, Supplier: Log in to see
Antigen
  • SETDB1
  • NV10069
  • fc07d03
  • si:ch211-81a5.6
  • wu:fc07d03
  • ESET
  • H3-K9-HMTase4
  • KG1T
  • KMT1E
  • TDRD21
  • AU022152
  • mKIAA0067
Epitope
AA 2-101
20
9
4
4
3
3
1
1
1
Reactivity
Human
89
34
20
10
Host
Mouse
66
23
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
74
45
20
11
8
7
6
5
3
1
1
Supplier
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Immunogen SETDB1 (NP_036564, 2 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4A3
Isotype IgG2a kappa
Specificity SETDB1 (NP_036564, 2 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SETDB1 (SETDB1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 143157 DA
Gene ID 12406
NCBI Accession NP_036564
Research Area Transcription Factors, Chromatin, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-SET Domain, Bifurcated 1 (SETDB1) (AA 2-101) antibody (ABIN394317) Immunofluorescence of monoclonal antibody to SETDB1 on HeLa cell (antibody concentrat...
Immunohistochemistry (IHC) image for anti-SET Domain, Bifurcated 1 (SETDB1) (AA 2-101) antibody (ABIN394317) anti-SET Domain, Bifurcated 1 (SETDB1) (AA 2-101) antibody (Image 2)
Background publications Mavaddat, Dunning, Ponder et al.: "Common genetic variation in candidate genes and susceptibility to subtypes of breast cancer." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 1, pp. 255-9, 2009 (PubMed).

Van Duyne, Easley, Wu et al.: "Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTR." in: Retrovirology, Vol. 5, pp. 40, 2008 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Gao, Yu, Bi et al.: "Akt/PKB interacts with the histone H3 methyltransferase SETDB1 and coordinates to silence gene expression." in: Molecular and cellular biochemistry, Vol. 305, Issue 1-2, pp. 35-44, 2007 (PubMed).

Ryu, Lee, Hagerty et al.: "ESET/SETDB1 gene expression and histone H3 (K9) trimethylation in Huntington's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 50, pp. 19176-81, 2006 (PubMed).