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Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D) (AA 1-92) antibody

Details for Product No. ABIN394324, Supplier: Log in to see
Antigen
  • EEF1D
  • EF1D
  • EF-1D
  • FP1047
  • 1700026P12Rik
  • 5730529A16Rik
  • AL023999
  • delta
Epitope
AA 1-92
6
4
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
65
22
21
Host
Mouse
58
7
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
40
16
13
11
10
9
4
3
3
1
1
Supplier
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Immunogen EEF1D (NP_115754, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4B12
Isotype IgG2a kappa
Specificity EEF1D (NP_115754, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EEF1D (EEF1D Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight NONE?! DA
Gene ID 12406
NCBI Accession NP_115754
Research Area Translation Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D) (AA 1-92) antibody (ABIN394324) EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expressio...
Immunohistochemistry (IHC) image for anti-Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D) (AA 1-92) antibody (ABIN394324) anti-Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D) (AA 1-92) antibody (Image 2)
Background publications Lei, Wang, Wei et al.: "Alternative expression and sequence of human elongation factor-1 delta during malignant transformation of human bronchial epithelial cells induced by cadmium chloride." in: Biomedical and environmental sciences : BES, Vol. 23, Issue 2, pp. 151-7, 2010 (PubMed).

Yang, Du, Wang et al.: "BMP-6 promotes E-cadherin expression through repressing deltaEF1 in breast cancer cells." in: BMC cancer, Vol. 7, pp. 211, 2008 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: , Vol. 3, pp. 89, 2007 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

De Bortoli, Castellino, Lu et al.: "Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8." in: BMC cancer, Vol. 6, pp. 223, 2006 (PubMed).