Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D) antibody

Antigen: Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D)

Clonality: Monoclonal (4B12)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394324

Additional Information

Alternative name: EEF1D

Gene ID: 1936

UniProt: NP_115754

Immunogen: EEF1D (NP_115754, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4B12

Description: Other names: EF-1D, FLJ20897, FP1047 eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: EEF1D (NP_115754, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: NONE?! DA

Comments:

Background: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in HepG2 ( Cat # L019V1 ). Detection limit for recombinant GST tagged EEF1D is approximately 1ng/ml as a capture antibody.

anti-Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D) antibody (Image 2)

Publications

Product: De Bortoli, Castellino, Lu et al.: "Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8." in: BMC cancer, Vol. 6, pp. 223, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Yang, Du, Wang et al.: "BMP-6 promotes E-cadherin expression through repressing deltaEF1 in breast cancer cells." in: BMC cancer, Vol. 7, pp. 211, 2008 (PubMed).

Lei, Wang, Wei et al.: "Alternative expression and sequence of human elongation factor-1 delta during malignant transformation of human bronchial epithelial cells induced by cadmium chloride." in: Biomedical and environmental sciences : BES, Vol. 23, Issue 2, pp. 151-7, 2010 (PubMed).