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Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D) antibody
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||EEF1D (NP_115754, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: EF-1D, FLJ20897, FP1047 eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||EEF1D (NP_115754, 1 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||NONE?! DA|
Background: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Translation Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
De Bortoli, Castellino, Lu et al.: "Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8." in: BMC cancer, Vol. 6, pp. 223, 2006 (PubMed).
Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).
Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).
Yang, Du, Wang et al.: "BMP-6 promotes E-cadherin expression through repressing deltaEF1 in breast cancer cells." in: BMC cancer, Vol. 7, pp. 211, 2008 (PubMed).
Lei, Wang, Wei et al.: "Alternative expression and sequence of human elongation factor-1 delta during malignant transformation of human bronchial epithelial cells induced by cadmium chloride." in: Biomedical and environmental sciences : BES, Vol. 23, Issue 2, pp. 151-7, 2010 (PubMed).
|Hosts||Rabbit (14), Mouse (6)|
|Reactivities||Human (20), Mouse (Murine) (8), Rat (Rattus) (5), Cow (Bovine) (2), Cat (Feline) (1), Chicken (1), Dog (Canine) (1), Pig (Porcine) (1), Rabbit (1)|
|Applications||ELISA (17), Western Blotting (WB) (17), Immunohistochemistry (IHC) (11), Immunofluorescence (IF) (3), Immunoprecipitation (IP) (3)|
|Epitopes||C-Term (2), N-Term (2), Center (1), Internal Region (1)|