Chromatin Accessibility Complex 1 (CHRAC1) antibody

Antigen: Chromatin Accessibility Complex 1 (CHRAC1)

Synonyms: YCL1, CHARC1, CHARC15, CHRAC15, 2410152E03Rik, 2810406L04Rik, CHRAC1, MGC142339, chrac1

Clonality: Monoclonal (4B8)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394329

Additional Information

Alternative name: CHRAC1

Gene ID: 54108

Immunogen: CHRAC1 (AAH15891, 1 a.a. ~ 132 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4B8

Description: Other names: CHARC1, CHARC15, CHRAC15, YCL1 chromatin accessibility complex 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CHRAC1 (AAH15891, 1 a.a. ~ 132 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 14711 DA

Comments:

Background: CHRAC1 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of CHRAC1 expression in transfected 293T cell line by CHRAC1 monoclonal antibody (M05), clone 4B8. Lane 1: CHRAC1 transfected lysate(14.7 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Collins, Poot, Kukimoto et al.: "An ACF1-ISWI chromatin-remodeling complex is required for DNA replication through heterochromatin." in: Nature genetics, Vol. 32, Issue 4, pp. 627-32, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).