DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56)

Synonyms: Noh61, D11Ertd619e, 2600001H07Rik, Ddx21, MGC93697, RH-II/Gu, MGC81243, noh61, zgc:110078, DDX56, MGC128435, DDX21, DDX26, NOH61

Clonality: Monoclonal (4C5)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394339

Additional Information

Alternative name: DDX56

Gene ID: 54606

UniProt: NP_061955

Immunogen: DDX56 (NP_061955, 450 a.a. ~ 548 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4C5

Description: Other names: DDX21, NOH61 DEAD (Asp-Glu-Ala-Asp) box polypeptide 56

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DDX56 (NP_061955, 450 a.a. ~ 548 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 61590 DA

Comments:

Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to DDX56 (AT1742a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody (M05), clone 4C5. Lane 1: DDX56 transfected lysate(62 KDa). Lane 2: Non-transfected lysate.

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56) antibody (Image 2)

Publications

Product: Lehner, Sanderson: "A protein interaction framework for human mRNA degradation." in: Genome research, Vol. 14, Issue 7, pp. 1315-23, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Andersen, Lam, Leung et al.: "Nucleolar proteome dynamics." in: Nature, Vol. 433, Issue 7021, pp. 77-83, 2005 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).