DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56) antibody

Details for Product No. ABIN394339
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Antigen
Synonyms Noh61, D11Ertd619e, 2600001H07Rik, DDX21, DDX26, NOH61, Ddx21, RH-II/Gu, MGC81243, noh61, zgc:110078, DDX56
Reactivity
Human
(17), (2), (1)
Host
Mouse
(11), (6)
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), Western Blotting (WB)
(17), (6), (5), (4), (3), (3), (2), (2)
Pubmed 5 references available
Catalog no. ABIN394339
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen DDX56 (NP_061955, 450 a.a. ~ 548 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4C5
Isotype IgG2a kappa
Specificity DDX56 (NP_061955, 450 a.a. ~ 548 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DDX56
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 61590 DA
Gene ID 12406
UniProt NP_061955
Research Area Translation Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56) antibody Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell (antibody concentration 10 µg/mL). Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody (M05), clone 4C5. Lane 1: DDX56 transfected lysate(62 KDa). Lane 2: Non-transfected lysate.
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56) antibody (2) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56) antibody (Image 2)
Background publications Lehner, Sanderson: "A protein interaction framework for human mRNA degradation." in: Genome research, Vol. 14, Issue 7, pp. 1315-23, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Andersen, Lam, Leung et al.: "Nucleolar proteome dynamics." in: Nature, Vol. 433, Issue 7021, pp. 77-83, 2005 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome...." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Hosts (11), (6)
Reactivities (17), (2), (1)
Applications (17), (6), (5), (4), (3), (3), (2), (2)
Epitopes (3), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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