Heart and Neural Crest Derivatives Expressed 2 (HAND2) (AA 135-217) antibody

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Antigen
  • DHAND2
  • Hed
  • Thing2
  • bHLHa26
  • dHand
  • dhand2
  • hand2
  • xhand2
  • hand2-b
  • Hand2'
  • dHAND
  • HAND2
  • hed
  • dhand
  • thing2
  • LOC100218345
  • AI225906
  • AI661148
  • Ehand2
  • Th2
  • zdHAND
  • zgc:103415
  • Hand2
  • heart and neural crest derivatives expressed 2
  • heart and neural crest derivatives expressed transcript 2
  • dHAND basic helix-loop-helix transcription factor
  • HAND2
  • Hand2
  • hand2-a
  • hand2-b
  • hand2
  • LOC100218345
  • DHAND
Alternatives
anti-Human Heart and Neural Crest Derivatives Expressed 2 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 135-217
33
12
10
10
10
8
7
6
3
1
1
Reactivity
Human
80
10
10
8
5
3
3
3
3
2
2
2
2
1
Host
Mouse
56
21
15
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
6
5
5
5
5
Application
Western Blotting (WB)
67
64
26
9
9
4
3
2
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen HAND2 (NP_068808, 135 a.a. ~ 217 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4D9
Isotype IgG2b kappa
Specificity HAND2 (NP_068808, 135 a.a. ~ 217 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name HAND2 (HAND2 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23666 DA
Gene ID 12406
NCBI Accession NP_068808
Research Area Transcription Factors, Signaling, Cell Structure
Pathways Tube Formation, Feeding Behaviour
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Heart and Neural Crest Derivatives Expressed 2 (HAND2) (AA 135-217) antibody (ABIN394348) Western Blot analysis of HAND2 expression in transfected 293T cell line by HAND2 mono...
Background publications Shen, Li, Shen, Wang, Liu, Guo, Song, Li: "Transcription factor HAND2 mutations in sporadic Chinese patients with congenital heart disease." in: Chinese medical journal, Vol. 123, Issue 13, pp. 1623-7, 2010 (PubMed).

Jugessur, Shi, Gjessing, Lie, Wilcox, Weinberg, Christensen, Boyles, Daack-Hirsch, Nguyen, Christiansen, Lidral, Murray: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Voth, Oberthuer, Simon, Kahlert, Berthold, Fischer: "Co-regulated expression of HAND2 and DEIN by a bidirectional promoter with asymmetrical activity in neuroblastoma." in: BMC molecular biology, Vol. 10, pp. 28, 2009 (PubMed).

Morikawa, Cserjesi: "Cardiac neural crest expression of Hand2 regulates outflow and second heart field development." in: Circulation research, Vol. 103, Issue 12, pp. 1422-9, 2008 (PubMed).

Han, Yi, Li, Olson: "Hand, an evolutionarily conserved bHLH transcription factor required for Drosophila cardiogenesis and hematopoiesis." in: Development (Cambridge, England), Vol. 133, Issue 6, pp. 1175-82, 2006 (PubMed).

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