DPH2 Homolog (S. Cerevisiae) (DPH2) (AA 125-236) antibody

Details for Product No. ABIN394378
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Antigen
Synonyms DPH2L2, 9130020C19Rik, AI467389, Dph2l2, id:ibd5058, zgc:162269
Epitope
AA 125-236
(10), (7), (7), (3), (2), (1), (1)
Reactivity
Human
(51), (17), (16), (14), (14), (12)
Host
Mouse
(30), (21)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(42), (19), (10), (9), (7), (6), (5), (5), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394378
450.00 $
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Immunogen DPH2 (NP_001375, 125 a.a. ~ 236 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5B10
Isotype IgG2a kappa
Specificity DPH2 (NP_001375, 125 a.a. ~ 236 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DPH2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52083 DA
Gene ID 12406
NCBI Accession NP_001375
Research Area Translation Factors, Signaling, Metabolism, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DPH2 Homolog (S. Cerevisiae) (DPH2) (AA 125-236) antibody Immunofluorescence of monoclonal antibody to DPH2 on HeLa cell (antibody concentration 10 µg/mL). DPH2 monoclonal antibody (M02), clone 5B10 Western Blot analysis of DPH2 expression in HeLa.
anti-DPH2 Homolog (S. Cerevisiae) (DPH2) (AA 125-236) antibody (2) anti-DPH2 Homolog (S. Cerevisiae) (DPH2) (AA 125-236) antibody (Image 2)
Background publications Schultz, Balasara, Testa et al.: "Cloning and localization of a human diphthamide biosynthesis-like protein-2 gene, DPH2L2." in: Genomics, Vol. 52, Issue 2, pp. 186-91, 1998 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Liu, Milne, Kuremsky et al.: "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9487-97, 2004 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Validation Images
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