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DPH2 Homolog (S. Cerevisiae) (DPH2) (AA 125-236) antibody

Details for Product No. ABIN394378, Supplier: Log in to see
Antigen
  • DPH2L2
  • 9130020C19Rik
  • AI467389
  • Dph2l2
  • id:ibd5058
  • zgc:162269
Epitope
AA 125-236
20
15
4
3
3
2
2
Reactivity
Human
53
10
9
3
2
1
Host
Mouse
37
16
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Western Blotting (WB)
47
26
6
6
6
6
2
Supplier
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Immunogen DPH2 (NP_001375, 125 a.a. ~ 236 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5B10
Isotype IgG2a kappa
Specificity DPH2 (NP_001375, 125 a.a. ~ 236 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DPH2 (DPH2 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52083 DA
Gene ID 12406
NCBI Accession NP_001375
Research Area Translation Factors, Signaling, Metabolism, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DPH2 Homolog (S. Cerevisiae) (DPH2) (AA 125-236) antibody (ABIN394378) Immunofluorescence of monoclonal antibody to DPH2 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-DPH2 Homolog (S. Cerevisiae) (DPH2) (AA 125-236) antibody (ABIN394378) anti-DPH2 Homolog (S. Cerevisiae) (DPH2) (AA 125-236) antibody (Image 2)
Background publications Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Liu, Milne, Kuremsky et al.: "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9487-97, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Schultz, Balasara, Testa et al.: "Cloning and localization of a human diphthamide biosynthesis-like protein-2 gene, DPH2L2." in: Genomics, Vol. 52, Issue 2, pp. 186-91, 1998 (PubMed).