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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) (AA 778-882) antibody

Details for Product No. ABIN394381, Supplier: Log in to see
Antigen
  • 2410015A15Rik
  • AI414901
  • chunp6913
  • DDX54
  • DP97
  • mgc2835
  • RGD1562539
  • zgc:111908
Epitope
AA 778-882
9
4
4
4
4
3
3
2
2
1
Reactivity
Human
37
3
2
1
1
1
1
Host
Mouse
22
17
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Western Blotting (WB)
29
21
9
9
9
8
6
1
Supplier
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Available images

Immunogen DDX54 (NP_076977, 778 a.a. ~ 882 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5B3
Isotype IgG2a kappa
Specificity DDX54 (NP_076977, 778 a.a. ~ 882 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DDX54 (DDX54 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 98595 DA
Gene ID 12406
NCBI Accession NP_076977
Research Area Translation Factors, Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) (AA 778-882) antibody (ABIN394381) DDX54 monoclonal antibody (M03), clone 5B3 Western Blot analysis of DDX54 expression ...
Immunohistochemistry (IHC) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) (AA 778-882) antibody (ABIN394381) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) (AA 778-882) antibody (Image 2)
Background publications Edelmann, Iphöfer, Akutsu, Altun, di Gleria, Kramer, Fiebiger, Dhe-Paganon, Kessler: "Structural basis and specificity of human otubain 1-mediated deubiquitination." in: The Biochemical journal, Vol. 418, Issue 2, pp. 379-90, 2009 (PubMed).

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Satoh, Nanri, Yamamura: "Rapid identification of 14-3-3-binding proteins by protein microarray analysis." in: Journal of neuroscience methods, Vol. 152, Issue 1-2, pp. 278-88, 2006 (PubMed).

Andersen, Lam, Leung, Ong, Lyon, Lamond, Mann: "Nucleolar proteome dynamics." in: Nature, Vol. 433, Issue 7021, pp. 77-83, 2005 (PubMed).

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).