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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) antibody

Details for Product No. ABIN394381
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Antigen
Synonyms DP97, AI414901, 2410015A15Rik, chunp6913, mgc2835, zgc:111908, RGD1562539, DDX54
Reactivity
Human
(27), (3), (1), (1), (1), (1), (1)
Host
Mouse
(15), (14)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(25), (15), (6), (6), (6), (6), (6), (2)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN394381
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen DDX54 (NP_076977, 778 a.a. ~ 882 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5B3
Isotype IgG2a kappa
Specificity DDX54 (NP_076977, 778 a.a. ~ 882 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DDX54
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 98595 DA
Gene ID 12406
UniProt NP_076977
Research Area Translation Factors, Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) antibody DDX54 monoclonal antibody (M03), clone 5B3 Western Blot analysis of DDX54 expression in A-431. Detection limit for recombinant GST tagged DDX54 is approximately 0.3 ng/mL as a capture antibody.
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) antibody (2) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Andersen, Lam, Leung et al.: "Nucleolar proteome dynamics." in: Nature, Vol. 433, Issue 7021, pp. 77-83, 2005 (PubMed).

Satoh, Nanri, Yamamura: "Rapid identification of 14-3-3-binding proteins by protein microarray analysis." in: Journal of neuroscience methods, Vol. 152, Issue 1-2, pp. 278-88, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Edelmann, Iphöfer, Akutsu et al.: "Structural basis and specificity of human otubain 1-mediated deubiquitination." in: The Biochemical journal, Vol. 418, Issue 2, pp. 379-90, 2009 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN394381
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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