E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody

Details for Product No. ABIN394384
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Antigen
Synonyms E2F-4, AI427446, 2010111M04Rik, E2F4, e2f-4
Reactivity
Human
(60), (28), (27), (13), (13), (13), (12), (1), (1)
Host
Mouse
(32), (27), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(48), (19), (17), (16), (14), (10), (8), (5), (4), (2), (1)
Pubmed 5 references available
Catalog no. ABIN394384
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen E2F4 (NP_001941, 211 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5B7
Isotype IgG1 kappa
Specificity E2F4 (NP_001941, 211 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name E2F4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 43960 DA
Gene ID 12406
UniProt NP_001941
Research Area Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody E2F4 monoclonal antibody (M01), clone 5B7 Western Blot analysis of E2F4 expression in A-549. Detection limit for recombinant GST tagged E2F4 is approximately 0.03 ng/mL as a capture antibody.
anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody (2) anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody (Image 2)
Background publications Lu, Dollé, Imholz et al.: "Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations." in: Journal of lipid research, Vol. 49, Issue 12, pp. 2582-9, 2008 (PubMed).

Bankovic, Stojsic, Jovanovic et al.: "Identification of genes associated with non-small-cell lung cancer promotion and progression." in: Lung cancer (Amsterdam, Netherlands), Vol. 67, Issue 2, pp. 151-9, 2010 (PubMed).

Garneau, Paquin, Carrier et al.: "E2F4 expression is required for cell cycle progression of normal intestinal crypt cells and colorectal cancer cells." in: Journal of cellular physiology, Vol. 221, Issue 2, pp. 350-8, 2009 (PubMed).

Cunningham, Vierkant, Sellers et al.: "Cell cycle genes and ovarian cancer susceptibility: a tagSNP analysis." in: British journal of cancer, Vol. 101, Issue 8, pp. 1461-8, 2009 (PubMed).

van Amerongen, Diehl, Novoyatleva et al.: "E2F4 is required for cardiomyocyte proliferation." in: Cardiovascular research, Vol. 86, Issue 1, pp. 92-102, 2010 (PubMed).

Hosts (32), (27), (2)
Reactivities (60), (28), (27), (13), (13), (13), (12), (1), (1)
Applications (48), (19), (17), (16), (14), (10), (8), (5), (4), (2), (1)
Conjugates (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (2), (2), (1), (1), (1)
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