Hepatocyte Specific Developmental Regulation 1 (HSDR1) antibody

Details for Product No. ABIN394386
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(5), (1)
(4), (2)
Clonality (Clone)
Monoclonal ()
Western Blotting (WB)
(6), (3)
Pubmed 5 references available
Catalog no. ABIN394386
Quantity 0.1 mg
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Immunogen ALF (NP_006863, 251 a.a. ~ 349 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5B9
Isotype IgG2b kappa
Specificity ALF (NP_006863, 251 a.a. ~ 349 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ALF
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52445 DA
Gene ID 12406
UniProt NP_006863
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000

Background: The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Hepatocyte Specific Developmental Regulation 1 (HSDR1) antibody ALF monoclonal antibody (M04), clone 5B9 Western Blot analysis of ALF expression in PC-12. Detection limit for recombinant GST tagged ALF is approximately 10 ng/mL as a capture antibody.
anti-Hepatocyte Specific Developmental Regulation 1 (HSDR1) antibody (2) anti-Hepatocyte Specific Developmental Regulation 1 (HSDR1) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Huang, Wang, Li et al.: "Involvement of ALF in human spermatogenesis and male infertility." in: International journal of molecular medicine, Vol. 17, Issue 4, pp. 599-604, 2006 (PubMed).

Howe, Mehmud, Saha et al.: "Transcription Factor IIA tau is associated with undifferentiated cells and its gene expression is repressed in primary neurons at the chromatin level in vivo." in: Stem cells and development, Vol. 15, Issue 2, pp. 175-90, 2006 (PubMed).

Kim, Li, Cui et al.: "Regulatory factor interactions and somatic silencing of the germ cell-specific ALF gene." in: The Journal of biological chemistry, Vol. 281, Issue 45, pp. 34288-98, 2006 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Hosts (4), (2)
Reactivities (5), (1)
Applications (6), (3)
Epitopes (2), (1)
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