T antibody

Details for Product No. ABIN394390
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(10), (1)
(6), (4)
Clonality (Clone)
Monoclonal ()
Western Blotting (WB)
(7), (6), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN394390
Quantity 0.1 mg
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Immunogen T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5C5
Isotype IgG2a kappa
Specificity T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 47443 DA
Gene ID 12406
UniProt NP_003172
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000

Background: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-T antibody T monoclonal antibody (M02), clone 5C5 Western Blot analysis of T expression in A-549. Detection limit for recombinant GST tagged T is approximately 0.3 ng/mL as a capture antibody.
anti-T antibody (2) anti-T antibody (Image 2)
Background publications Bondue, Lapouge, Paulissen et al.: "Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification." in: Cell stem cell, Vol. 3, Issue 1, pp. 69-84, 2008 (PubMed).

Oakley, Fuhrer, Seethala: "Brachyury, SOX-9, and podoplanin, new markers in the skull base chordoma vs chondrosarcoma differential: a tissue microarray-based comparative analysis." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 12, pp. 1461-9, 2008 (PubMed).

Yang, Ng, Alcorta et al.: "T (brachyury) gene duplication confers major susceptibility to familial chordoma." in: Nature genetics, Vol. 41, Issue 11, pp. 1176-8, 2009 (PubMed).

Docherty, Kovas, Petrill et al.: "Generalist genes analysis of DNA markers associated with mathematical ability and disability reveals shared influence across ages and abilities." in: BMC genetics, Vol. 11, pp. 61, 2010 (PubMed).

Jambhekar, Rekhi, Thorat et al.: "Revisiting chordoma with brachyury, a \new age\" marker: analysis of a validation study on 51 cases."" in: Archives of pathology & laboratory medicine, Vol. 134, Issue 8, pp. 1181-7, 2010 (PubMed).

Hosts (6), (4)
Reactivities (10), (1)
Applications (7), (6), (2), (2), (1)
Epitopes (2), (1), (1), (1)
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