|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: MGC104817, TFT T, brachyury homolog (mouse)|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||47443 DA|
Background: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
|T monoclonal antibody (M02), clone 5C5 Western Blot analysis of T expression in A-549 ( Cat # L025V1 ). Detection limit for recombinant GST tagged T is approximately 0.3ng/ml as a capture antibody. anti-T antibody (Image 2)|
Bondue, Lapouge, Paulissen et al.: "Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification." in: Cell stem cell, Vol. 3, Issue 1, pp. 69-84, 2008 (PubMed).
Oakley, Fuhrer, Seethala: "Brachyury, SOX-9, and podoplanin, new markers in the skull base chordoma vs chondrosarcoma differential: a tissue microarray-based comparative analysis." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 12, pp. 1461-9, 2008 (PubMed).
Yang, Ng, Alcorta et al.: "T (brachyury) gene duplication confers major susceptibility to familial chordoma." in: Nature genetics, Vol. 41, Issue 11, pp. 1176-8, 2009 (PubMed).
Docherty, Kovas, Petrill et al.: "Generalist genes analysis of DNA markers associated with mathematical ability and disability reveals shared influence across ages and abilities." in: BMC genetics, Vol. 11, pp. 61, 2010 (PubMed).
Jambhekar, Rekhi, Thorat et al.: "Revisiting chordoma with brachyury, a "new age" marker: analysis of a validation study on 51 cases." in: Archives of pathology & laboratory medicine, Vol. 134, Issue 8, pp. 1181-7, 2010 (PubMed).